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Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Autónoma de Yucatán

1999, Number 1

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Rev Biomed 1999; 10 (1)

Measurement of L-canavanine by reverse phase high performance liquid chromatography and by an spectrofotometric method.

Capetillo-Leal CM, Reyes-Ramírez RR, Sandoval-Castro CA

Language: Spanish
References: 8
Page: 17-22
PDF size: 30.30 Kb.


ABSTRACT

Introduction. In order to study toxicity and metabolic effects of canavanine in animals or plants a quick, sensitivy and specific method is required. Material and methods. A reverse phase high performance liquid chromatography (HPLC), using DABS-Cl as derivatizing agent, was developed and the results compared with those obtained by colorimetry using the Rosenthal method. Five canavalia samples were used to validate the method. Results and discusion. Canavanine determination by HPLC was free from interference with other aminoacids. No significant diferences were found on canavanine concentration in Canavalia ensiformis grains as determined by both methods. It was concluded that colorimetric methods may be a first choice option for a rapid screening process. Due to the high cost involved in the HPLC technique, this method should be left for cases when there is interference with metabolites sample is known to exist using the colorimetric technique.


REFERENCES

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  3. 3.- D’Mello JPF, Acamovic T, Walker AG. Nutritive value of jack beans (Canavalia ensiformis (L.) DC) for young chicks. Trop. Agric. (Trinidad) 1985; 62:145-50.

  4. 4.- Oropeza C, Alpizar L, Loyola-Vargas VM, Quiroz J,Scorer K. Determination of L-canavanine and L-canaline in plant tissues by high performance liquid chromatography. J Chromatography. 1988; 456:405-409.

  5. 5.- Weissberger LT, Armstrong MK. Canavanine analysis of Alfalfa extracts by high performance liquid chromatography using pre-column derivatization. J Chromatographic Sci. 1984; 22:438-440.

  6. 6.- Acamovic T; D’Mello F. HPLC analysis of Canavanine and Canaline in Canavalia Ensiformins, and in Excreta and Serum Chicks. J Sci Food Agric 1990; 50:63-70.

  7. 7.- Knecht R, Chang J. Liquid chromatographic determination of amino acids after gas-phase hydrolysisi and derivatization with (Dimethylamino)azobenensulfonyl Chloride. Anal Chem 1986; 58:2375-9.

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