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Revista Salud Pública y Nutrición

Coordinación General de Investigación de la Facultad de Salud Pública y Nutrición y la Dirección General de Sistemas e Informática de la Universidad Autónoma de Nuevo León
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2001, Number 2

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Rev Salud Publica Nutr 2001; 2 (2)

Evaluación de la sensibilidad y especificidad de dos métodos de baciloscopia

Flores AA, Llaca DJM, Ramos PEG
Full text How to cite this article

Language: Spanish
References: 18
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Key words:

Tuberculosis, direct microscopic exam, cultive.

ABSTRACT

The direct microscopic exam is the most useful tool in the diagnosis, control and treatment of the tuberculosis, mainly in countries in development. The purpose was to demonstrate if difference existed in the sensibility and specificity in the direct microscopic exam of extensive area (BACEX) with regard to that of reduced area (BACRED). it was a study carried out in 100 patients' that went to the Hospital University Doctor José Jose Eleuterio González UANL (Monterrey, N.L, México). The sputum should have quality and aspect, content in a plastic recipient of high density, translucent and sterile, it was standardized the observation area, the quantity of sample and the observers' experience. 58% was of the masculine and, feminine 42%; age average was of 49 years (range 11 at 103), 76% had the negative cultivation, positive 23% for Mycobacterium tuberculosis, 1% for Mycobacterium chelonae. The BACEX observed by inexpert personal had a sensibility of 45% (IC95 0.25, 0.65) and specificity of 93% (IC95 0.88, 0.98), for the BCRED the sensibility was of 54% (IC95 0.43, 0.65) and specificity of 93% (IC95 0.88, 0.98); The BACEX and the BACRED observed by the fairly expert personal, the sensibility was of 75% (IC95 0.58, 0.92) and specificity of 98% (IC95 0.95, 1.00); for the expert, the sensibility it was of 79% (IC95 0.63, 0.95) and specificity of 100%. he/she was not significant difference between the sensibility and specificity of both methods, the experience was a decisive factor that influenced in the value diagnosis of the direct microscopic exam, all laboratory that it carries out direct microscopic exam it will establish quality intern's controls and external, the inexpert personal will receive training.


REFERENCES

  1. WHO. 2001 Global tuberculosis Control. WHO Report 2001.

  2. Dirección General de Epidemiología. Secretaría de Salud (www.ssa.gob.mx)

  3. Raviglione MC, P Sudre, HL Rieder, S Spinaci, and A Kochi. 1993 Secular trenas of tuberculosis in Western Europe. Bull WHO; 71:297-306.

  4. OPS 1988. Control de la Tuberculosis. Manual sobre Métodos y Procedimientos para Programas Integrados. OPS/OMS Publicación Científica Núm. 498

  5. Toman, K.,1980. Tuberculosis .Detección de Casos y Quimioterapia. Preguntas y Respuestas, OPS/OMS Publicación Científica Núm. 392, pp 3-73

  6. OPS 1988a. Manual de Normas y Procedimientos Técnicos para la Bacteriología de la Tuberculosis, Parte I La Muestra. El Examen Microscópico, Nota Técnica Núm. 26/Rev. I

  7. OPS 1988b. Manual de Normas y Procedimientos Técnicos para la Bacteriología de la Tuberculosis, Parte II. El Cultivo , Nota Técnica Núm. 27/Rev. I (7)

  8. Toman, K, op cit..

  9. OPS 1988b., op cit.

  10. Koneman, W Elmer., Allen D Stephen., Sommers, y M Herbert. 1985 Diagnóstico Microbiológico Ed. Panamericana pp 403-426

  11. Richmond JY; and RW McKinney., 1993 Biosafety in microbiological and biomedical laboratories. Publication No. CDC 93-8395. US Dept of Health and Human Services, Public Health Service, C for Disease Control and Prevention, National Institutes of Health, ed3, , pp 93-96.

  12. OPS 1988 a op.cit.

  13. OPS 1988b., op cit.

  14. Couchot K, and R Talbot,., 1996 Direct DNA Probe of MB/Bact Mycobacterial Culture System Bottles for Mycobacterium avium complex and Mycobacterium Tuberculosis complex Annual Meeting of the American Society for Microbiology pp 55-58

  15. Wanger, A., R. Clarck, J. Bua, A. Eduards, and J. Ho, 1996. Comparison of MB/Bact and conventional methods for detection of Mycobacterium species. Annual Meeting of the American Society for Microbiology pp 73-78

  16. OPS 1988b., op cit.

  17. Koneman, W Elmer et. al. op cit

  18. Collins, C.H., J.M.. Grense, M.D. Yates 1997. Tuberculosis Bacteriology: Organization an Practice Ed. Buttetrworth Heinemann Second Edition pp 1-56




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Rev Salud Publica Nutr. 2001;2