1994, Number 2
Use of beer as an alternative supplement for factor V in the in vitro growth of Actinobacillus pleuropneumoniae> factor V dependent
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Text ExtractionThe present study tested the use of beer as a substitute for factor V in the in vitro growth of Actinobacillus pleuropneumoniae serotypes 1,3,5,7,9,11 and 12 factor V-dependent. A culture kinetics of A. pleuropneumoniae serotype 1 grown on brain and heart infusion broth, supplemented with either 0.01% nicotine-adeninedynucleotide (BHI-NAD) or 20% beer (BHI-C), was compared. Generation time was shorter and adaptation phase larger on BHI-C; however, at the end of the culture, equivalent quantities of microorganisms were obtained in both media. During the kinetics, excretion of haemolysin was evaluated in both media, and it was observed that beer did not inhibit synthesis and excretion of this important toxin. It is concluded that beer is an economic and easy to handle alternative for factor V in the culture of A. pleuropneumoniae.
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