Santillán BJG, Núñez DCN, Morales ÁE, Mejía GE

Language: Spanish
References: 6
Page: 278-282
PDF size: 76.91 Kb.

ABSTRACT

Background: The analysis of cell counting in body fluids has been traditionally performed in a manual way, using the conventional or manual method. This process involves a series of steps that contribute to imprecision and excessive waste of time. XE-2100 is an analyzer that works through the principle of fluorescence flow cytometry, which presents a program designed for cell counting in body fluids. Objective: To determine the precision of XE-2100 in clinical samples and to evaluate the concordance and correlation for leukocyte counting between the analyzer and the conventional (manual) method. Methodology: Precision: This study used six clinical samples of fluid coming from peritoneal dialysis. From these samples, ten repetitions of each mentioned method were carried out, the same as with any body fluid samples. Concordance and correlation: Forty-two samples of fresh body fluid samples were analyzed and subjected to a cytological examination. The counting of leukocytes was carried out by means of the conventional method and with the XE-2100 device or equipment. The statistical test used was Pearson correlation test. Results: Precision: It was observed a variation coefficient of 8.76%. This observed coefficient was lower than the one reported by the manufacturer, who states that the coefficient should be less than the 30%. Correlation: The correlation coefficient for the leukocyte counting between the XE-2100 device and the conventional or manual method was of r = 0.941, which is significant for a value of p ‹ 0.041. Besides this, it was obtained an equation of regression of y = 110.6302 + 0.7146 x. Conclusions: The employed device or equipment XE–2100 may be used to perform leukocyte counting in corporal or body fluids with a lot of security and confidence or trust.

REFERENCES

1. Paramothayan NS, Barron J. New criteria for the differentiation between transudates and exudates. J. Clin Pathol 2002; 55: 69-71.

2. Penders J, Fiers T, Dhondt AM, Claeys G, Delanghe JR. Automated flow cytometry analysis of peritoneal dialysis fluid. Nephrol Dial Transplant 2004; 19: 463-468.

3. Henry JB. Diagnóstico y Tratamiento clínico por el Laboratorio. 9a ed. España: Editorial Masson; 2000. p. 459-487.

4. Gómez-Gaviño V, Jímenez-López C, Vivar-Guzman NP, Sánchez-Rodríguez MA. Comparación del citómetro UF100i con el sistema Kova y el método convencional para el conteo de leucocitos y eritrocitos en orina. Bioquimia 2008;(33)2: 51-58.

5. Jonge R, Brouwer R, Smit M, Frankrijer-Merkerstijn, Dolhain RJEM, Hazes JMW, Van Toorenenbergen, Lindemans J. Automated counting of white blood cells in synovial fluid. Rheumatology 2004; 43: 170-173.

6. Diario Oficial de la Federación. NOM-166-SSA1-1997. Para la Organización y Funcionamiento de los laboratorios clínicos. Jueves 13 de enero del 2000.