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Revista Latinoamericana de Microbiología

2008, Number 3-4

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Microbiología 2008; 50 (3-4)

West Nile Virus detection by RT-PCR from mosquitoes in a locality of Baja California, Mexico

Medina GE, Sandoval EA, Rentería TB, López G, Mora A, Pujol LC

Language: English
References: 14
Page: 83-86
PDF size: 59.20 Kb.


ABSTRACT

Background: West Nile virus (WNV) is an important public health flavivirus transmitted to humans by infected mosquitoes. Transmission involves birds and primarily Culex sp. mosquitoes, with humans as incidental hosts but with severe disease in some cases. Methods: A total of 2,333 mosquitoes distributed in 92 pools were sampled in a locality of Mexicali Valley, Baja California, Mexico, between September 2005 and August 2006 using CO2traps and aspiration tubes. Mosquito samples were identified, divided into groups by species and month of sampling and tested by reverse transcription-polymerase chain reaction (RT-PCR) in order to estimate the relative abundance of each mosquito species per month and the WNV infection rate respectively. Results: A total of seven mosquito species were identified and three of these were found to be infected with WNV: one in October of 2005 corresponding to Aedes vexans; two more in November 2005 and April 2006 corresponding to Culex pipiens/quinquefasciatus; and one more in May 2006 corresponding to Culex tarsalis. Conclusion: The risk of WNV infection in human population in the study area exists, and actions to control mosquito populations for veterinary and public health authorities who conduct surveillance programs in the region are necessary, principally in spring and autumn.


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