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2008, Number 3-4

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Microbiología 2008; 50 (3-4)

A rapid DNA extraction method from mycelium which is suitable for PCR

Conde-Ferráez L, Grijalva-Arango R, James-Kay AC
Full text How to cite this article

Language: English
References: 5
Page: 87-89
PDF size: 0. Kb.


Key words:

Aislamiento de ADN, hongos filamentosos, homogenizador, ITS, arena de cuarzo.

ABSTRACT

We describe a protocol that is rapid, simple and efficient for the isolation of DNA from fungi. It does not require liquid nitrogen and uses disposable homogenizers in eppendorf tubes. Because of its simplicity, this method is appropriate for simultaneous processing of a large number of samples and for population analyses with PCR techniques.


REFERENCES

  1. Dellaporta, S., J. Wood & J.B. Hicks. 1983. A plant DNA minipreparation: version II. Plant MoI Biol Rept 1:19-21.

  2. Johanson, A. 1997. Detection of Sigatoka Leaf Spot Pathogens of Banana by the Polymerase Chain Reaction. Natural Resources Institute, Chatham U.K.

  3. León, G., L. Holuigue, & X. Jordana. 2007. Mitochondrial Complex II is essential for gametophyte development in Arabidopsis. Plant Physiology 143: 1534-1546.

  4. Mahuku, G.S. 2004. A simple extraction method suitable for PCR-based analysis of plant, fungal, and bacterial DNA. Plant Molecular Biology Reporter 22: 71-81

  5. White, T.J., T. Bruns, S. Lee & J. Taylor. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics, p 315-322. In M.A. Innis, D.H. Gelfand, J.J. Sninsky & T.J. White (Eds). PCR protocols. Academic Press, London.




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C?MO CITAR (Vancouver)

Microbiología. 2008;50