2007, Number 2
Sporotrichosis epidemiology in Huauchinango, Puebla
Munguía PR, Romo LY, Castañeda RE, Velázquez EMC, Espinosa TA
Full text
Language: Spanish
References: 17
Page: 38-43
PDF size: 181.31 Kb.
ABSTRACT
Introduction. Sporotrichosis is an unstudied micosis subcutaneous in some places in Mexico with increased cases not diagnosticated and unknown environmental sources of transmission.Aim: To know the frecuency, clinic type of sporotrichosis and environmental distribution of Sporothrix schenckii in Huauchinango, Puebla-Mexico.
Methodology. We studied ten individuals with sporotrichosis from Huauchinango, Puebla-Mexico. In each one of them was applied intradermoreaction with metabolic sporotricine doing readings 48 hours later inoculation and following the conventional criteria. Samples of the lesions was done at the same time, they were cultured in Sabouraud glucose agar with and without antibiotics. We processed 100 environmental samples from plants and soil for the isolation of Sporothrix schenckii briefly: we soaked one gram of each sample (plant or soil) and we done three dilutions 1:10, 1:100, 1:1,000; each dilution was cultured in Sabouraud glucose agar with antibiotics, incubated at 28 °C during 20 days. The fungi identification was done by macroscopic and microscopic analysis of the mycelial and yeast-like phases, as well as the reproduction of animal sporotrichosis in balb/c mice.
Results. We detected ten sporotrichosis patients from Huauchinango, one of them was from Hidalgo and the last nine were from Ahuacatlán, Puebla. Three patients presented sporotrichosis fixed clinic type and seven linfangitic type. The individuals more affected were students and farmers. The illness evolution was between 1 to 13 years. The susceptible edges for sporotrichosis were 19 to 78 years. Three men and seven women manifested sporotrichosis. We obtained three Sporothrix schenckii strains, one strain from soil associated to pine, other strain from rosebush and other one strain from gladiolus.
Conclusion. Sporotrichosis was endemic in Huauchinango, Puebla-Mexico with ten new clinic cases. The clinic form more frequent of Sporotrichosis was the linfangitic predominating in the female than in the masculine sex. Three Sporothrix schenckii strains were isolated of soil; this could be the main source of infection transmision.
REFERENCES
Espinosa Texis A, Romo Lozano Y y Rivera Tapia JA. “Aislamiento de agentes de micosis subcutáneas a partir de agua, suelo y plantas”, en Rocha-Gracia R del C, Y Martínez-Laguna y JF López-Olguín (coords.), Temas de actualidad en microbiología, ambiente y salud, Benemérita Universidad Autónoma de Puebla, Puebla, México, 2002, pp. 197-212.