Reyna FJ, Villeda GG, Ortiz IFJ, Morales MI, Flores MS

Language: Spanish
References: 6
Page: 54-58
PDF size: 65.08 Kb.

ABSTRACT

Introduction. Detection of micoplasmas by fast a test is an alternative diagnosis in sites where the laboratory test is not available.
Methods. Means a prospective cohort study we studied clinical endocervical samples and seminal samples of women and men with infertility diagnosis. To both we inoculated the clinical samples in urea and arginine broths, as well as the Mycofast^® commercial kit.
Sensitivity, specificity, and predictive values were analyzed; we compared the fast test vs. culture broths.
Results. Twenty-five couples were studied—we found sentitivities over 70%, and predictive positive values lower than 40%. The predictive negative value was greater than 90%.
Conclusion. The commercial fast test is recommended to be used in places without microbiological infrastructure to detect mycoplasmas. The culture broth or molecular biology is still the best diagnostic method however.

REFERENCES

1. Broittman LN, Floy MC, Johnson AC. “Comparison of commercially available media for detection and isolates of Ureaplasma urealyticum and Mycoplasma hominis”. Journal of Clinical Microbiology 1992; 1335-1337.

2. Ramírez IC, Casanova RG, Menocal TG, Ortiz IFJ. “Prevalencia de la infección cervicovaginal por Mycoplasma hominis y Ureaplasma urealyticum en pacientes ginecológicas del Instituto Nacional de Perinatología”. Enfermedades Infecciosas y Microbiología 2004; 24.

3. Rojas RJ, Bravo GC, Tapia SR. “Las bacterias como causa de infertilidad masculina”. Instituto de Medicina Reproductiva y Andrología. México, DF. Disponible en www. angelfire.com/ex2/imra/infertilidad.htm

4. Casanova RG, Ortiz IFJ, Reyna FJ. Infecciones de transmisión sexual. 1ª ed., México, Alfil, 2004, 149-162.

5. Renaudin H, Bébéar C, Bébéar M. “Evaluation of commercial kit for antibiotic susceptibility testing of genital mycoplasmas”. Laboratoire de Bacteriologie ,EA 3671, Université Victor Segalen, Bordeaux 2, Bordeaux, Francia.

6. Koneman WE, Stephen DA, William M, Janda WM, Schreckenberger CP. 3a,ed., Diagnóstico microbiológico, 3a ed., Panamericana, México, 1999, 833-866.