Taboada SCS, López UAC, Jiménez PF, Raya SSB, Ortiz MLA, Basurto MP

Language: Spanish
References: 10
Page: 119-124
PDF size: 233.59 Kb.

ABSTRACT

Background: Acute perforation of the tympanic membrane is caused by infections or trauma. Surgical reconstruction techniques use different materials and often require general anesthesia, with the resulting morbidity. Phenytoin has been used topically in the healing of varicose ulcers by its effect on epithelial proliferation, besides the ease of application.
Objective: To determine the effectiveness of topical phenytoin in the closure of eardrum perforations in Wistar rats.
Material and method: We designed an experimental, prospective and longitudinal clinical trial of 30 male Wistar rats that underwent a bilateral eardrum perforation in the lower quadrants. The rats were divided randomly into six groups (group A: control, perforated ears only, no medication was applied; group B: 100 mg of phenytoin in 1 mL of methylcellulose; group C: 50 mg/1 mL of methylcellulose; group D: 25 mg/1 mL of methylcellulose; group E: 12.5 mg/1 mL of methylcellulose; group F, only saline solution applied in perforation).
Results: In the control group, one of the perforations healed in the first examination, two in the second examination, five healed in the last examination and two never healed. In group 2 (saline), none of the perforations healed in the first examination, three healed in the second examination, six in the third examination and one never healed. In the third group, 9 perforations healed in the first examination and one in the second. In the fourth group, eight perforations healed in the first examination and two in the second. In the fifth group, 10 perforations healed in the first examination. In the sixth group, three perforations healed in the first examination, two in the second examination, three in the third examination and two perforations never healed.
Conclusions: Experimental perforations of the tympanic membrane in rats healed spontaneously over a period of two weeks in 80% of cases. Phenytoin accelerates the healing according to the dose applied topically. The dose of 50 mg has the best effect; on the contrary, the dose of 100 mg produced the worst results.

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