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Instituto Nacional de Salud Pública

2012, Number 1

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salud publica mex 2012; 54 (1)

Evaluation of four methods for detecting methicillin-resistant Staphylococcus aureus isolates from clinical specimens at a regional hospital in Mexico

Acosta-Pérez G, Rodríguez-Ábrego G, Longoria-Revilla E, Castro-Mussot ME

Language: Spanish
References: 18
Page: 1-6
PDF size: 110.64 Kb.


ABSTRACT

Objetive.To estimate the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in clinical isolates and to compare different methods for detection of MRSA in a lab with limited available personnel and resources. Material and Methods. 140 Staphylococcus aureus strains isolated from patients in several departments were assayed for β-lactamase production, MIC-Vitek 2 oxacillin, ChromID MRSA, disk diffusion in agar for cefoxitin 30 µg and PBP2a detection. The results of conventional tests were compared with the “gold standard” PCR test for mecA gene. Cohen´s kappa index was also calculated in order to evaluate the intra assay agreement between the used methods. Results. The found prevalence was 90.7%. Sensitivity and specificity were: disk diffusion for cefoxitin 97 and 92% respectively, MIC Vitek 2-XL 97 and 69%, ChromoID MRSA 97 and 85%, and PBP2a detection 98 and 100%. Conclusions. All methods are very good for detecting MRSA, choosing a method to use will depend on each laboratory infrastructure.


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