2012, Number 01
Breast cancer association with the polymorphisms T-66G and G-156GG SPP1 gene and levels serum osteopontin
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ABSTRACTBackground: Recently osteopontin was associated with breast cancer, but has not studied the risk conferred by polymorphic variants of the gene encoding SPP1.
Objective: To determine the association between breast cancer, polymorphisms T-66G and G-156GG SPP1 gene, haplotypes formed by them, and serum osteopontin in women in northern Mexico.
Method: A case-control study with 150 cases and 225 controls of patients treated at the Cancer Center of Durango State. All participants gave their informed consent. The determination of the polymorphisms was performed by DNA fused high resolution and osteopontin by ELISA. Statistical analysis was performed using Haploview and Episheet.
Results: for G-156GG polymorphism, the model showed allele odds ratio (OR) of 2.25, and the dominant model of 2.74 RM. The value of standardized linkage disequilibrium of 0.15. The GG haplotype -66G/-158 -66T/-158GG and MRI showed 1.84 and 4.70, respectively. The concentration of osteopontin was higher in cases (p ‹0.001). No association of alleles and genotypes with concentration of osteopontin.
Conclusions: These results suggest that allele-156GG SPSS1 increases the risk of cancer. Osteopontin is elevated in patients with breast cancer, but no association with the alleles or genotypes studied. This is the first study in Mexican population of polymorphisms in the promoter region of SPP1 contributes to characterize the genetic structure of our population.
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