Kably AA, Ruiz AJ, Carballo ME, Garzón NJA, Karchmer KS

Language: Spanish
References: 15
Page: 11-16
PDF size: 78.20 Kb.

ABSTRACT

Objective: We sought to evaluate the follicular microenvironment and its relation with quality and type of ovocyte. Type of study: Prospective, observational, and analytical. Material and methods: One hundred thirty six patients from the in vitro Fertilization Program at the Hospital Angeles de las Lomas in Mexico City were included. We obtained follicular liquid from each patient during ovular capture and determined intrafollicular estradiol levels by fluorescence, and progesterone and testosterone concentration by chemoluminescence. Ovocyte quality was calculated based on presence of polar body, perivitelline space morphology, and cytoplasmatic inclusions. Statistical analysis was carried out with Pearson P and Student t tests with 95% confidence interval (95% CI). Results: Average patient age was 33.8 ± 0.5 years. Number of captured ovocytes captured was 10.53 ± 7.14 per patient. Estradiol and progesterone follicular levels did not show statistical correlation with ovocyte quality and maturity. Testosterone levels ‹ 1,400 ng/mL presented a greater amount of mature ovoctyes with excellent morphologic quality. Estradiol/progesterone index ‹ 100 was associated with better ovocyte quality (59.26 vs 35.71%). Estradiol/testosterone relationship › 900 was associated with mature ovocytes (77.8 vs 59.6%). No relation was observed between follicular hormone levels and percentage of fertilization. Conclusions: Intrafollicular hormonal environment is fundamental in ovocyte development; low testosterone levels are related with better quality and ovular maturity. Estradiol/progesterone relationship ‹ 100 is associated with better ovocyte quality.

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