2003, Number 3
Spermatic cryopreservation; influence over the rate of survival and its future repercussion
Góngora A, Capilla G, Trejo P
Full text
Language: Spanish
References: 11
Page: 133
PDF size: 51.65 Kb.
ABSTRACT
Introduction: The present work consisted of comparisons of the ratio of survival for two cryopreservation pool samples: a pool with ‹ 5 years of cryopreservation and a pool with › 5 years of cryopreservation, to evaluate whether there was any apparent damage in the spermatic cells. Methods: We analyzed 100 samples of human seminal liquid obtained for cryopreservation. Evaluation was carried out according to World Organization Health criteria (WHO). Samples were thawed in a water bath at 37°C ± 1°C for 20 min; subsequently, we carried out a microscopic evaluation for the two types of samples. Results: We found that the more time that a spermatic sample is maintained under liquid nitrogen, the more damage and less motility of sperm cells occurs. We made an estimation of the difference between the two populations with a 95% confidence intervals and found a true but not significant difference (p = 0.2–0.12). We can assume that the majority of cryopreserved sperm samples will have a reduction in mobility during the cryopreservation process, even though the majority is based on individual differences. This has led to development and use of cryoprotectors with extenders to control alterations in cells and solvents to obtain samples with better quality and longer storage time. The difference is minimum in motility; however the amount of motile sperms suggests good results, i.e, samples with sperm counts above 10 millions/mL.REFERENCES
Larson JM, McKinney KA, Mixon BA, Burry KA, Wolf DP. An IUI-ready cryopreservation method compared with sperm recovery after conational freezing and post thaw processing. Presented at the 5th Annual Meeting of the American Society of Reproductive Medicine. Washington, D.C., USA. October 1995. Department of Obstetrics/Gynecology, Oregon Health Sciences University, USA.