2011, Number 4
Rev Hematol Mex 2011; 12 (4)
Analysis of bcr-abl molecular rearrangement in cuban patients with presumptive diagnostic chronic myeloid leukemia
Casanueva-Calero K, Pantaleón-Florido G, Ruiz-Noa Y, Mato-Luis J, Quesada-Dorta M, Carnot-Uria J
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ABSTRACTBackground: Chronic myeloid leukemia (CML) represents 15% of leukemias in adults. Its early recognition from other myeloproliferative syndromes is fundamental for beginning treatment and increasing chances for a favorable outcome. Detection of Philadelphia chromosome or the subjacent BCR-ABL rearrangement is necessary to confirm the diagnosis of CML. But BCR-ABL determination by reverse transcription and polymerase chain reaction is a more sensitive method.
Material and method: Here we applied a qualitative variant of that methodology for BCR-ABL detection in 27 patients with CML diagnosis.
Results: Out of total patients 93% was positive to BCR-ABL, confirming the diagnosis of CML. BCR-ABL transcripts b3a2, b2a2, and e1a2 were found in 60%, 32% and 8% of positive patients, respectively. Gender analysis revealed a greater presence of b2a2 in women (40%) than in men (27%). Variant e1a2 was exclusively detected in the latter. No co-expression of the transcripts tested was found.
Conclusions: These results support the diagnostic utility of the methodology employed and warrant the evaluation of a higher amount of CML patients for corroborating the genetic frequencies encountered and assessing their relationship with prognostic parameters.