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2008, Number 4

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Rev Educ Bioquimica 2008; 27 (4)

La fluorescencia de la clorofila a como herramienta en la investigación de efectos tóxicos en el aparato fotosintético de plantas y algas

González MS, Perales VH, Salcedo AMO
Full text How to cite this article

Language: Spanish
References: 9
Page: 119-129
PDF size: 1109.27 Kb.


Key words:

photosynthesis, chlorophyll a fluorescence, plants, algae, toxic effects.

ABSTRACT

Chlorophyll a fluorescence emission analysis of photosystem II from the photosynthetic apparatus of terrestrial and aquatic plants and algae makes possible to characterize the effects and modes of action of different kinds of environmental stress (temperature, drought, high irradiance, salinity, flooding), severalwater pollutants such as heavy metals, herbicides, detergents, and a variety of air pollutants, as well. It may be of great help for identification of toxic pollutants and their sources. In addition, this method of analysis can to be applied to plants and algae in situ, and in vivo or to isolated chloroplasts, is not invasive neither destructive and it is a fast and sensitive technique.


REFERENCES

  1. Maxwell K, Johnson GN (2000) Chlorophyll fluorescence. A practical guide. J Exp Bot 51: 659-668.

  2. Strasser RJ, Srivastava A, Tsimilli-Michael M (2000) The fluorescence transient as a tool to characterize and screen photosynthetic samples. En: Probing Photosynthesis, Mechanism, Regulation, Adaptation. Editores: Yunus M, Pathre U, Mohanty P. Taylor & Francis. pp 445-483.

  3. Brack W, Frank H (1998) Chlorophyll a fluorescence: Atool for the investigation of toxic effects in the photosynthetic apparatus. Ecotoxicol Environ Saf. 40:34-41.

  4. Appenroth KJ, Stöckel J, Srivastava A, Strasser RJ (2001) Multiple effects of chromate on the photosynthetic apparatus of Spirodela polyrhiza as probed by OJIP chlorophyll a fluorescencemeasurements. Environ Pollut 115:49-64.

  5. Bolhàr-Nordenkampf HR, ÖquistG (1993) Chlorophyll fluorescence as a tool in photosynthesis research. En: Photosynthesis and Production in a Changing Environment: A field and Laboratory Manual. Editores: Hall DO, Scurlock JMO, Bolhàr-Nordenkarpf HR, Leegood RC, Long SP. Chapman & Hall pp 193-206

  6. Strasser BJ, Strasser RJ (1995) Measuring fast fluorescence transients to address environmental questions: En Photosynthesis: FromLight to Biosphere, Vol 5: Editor: Mathis P Kluwer Academic pp 977-980.

  7. Rohácek K, Barták M (1999) Technique of the modulated chlorophyll fluorescence: basic concepts, useful parameters, and some applications. Photosynthetica 37:339- 363.

  8. Müller P, Li Xiao-Ping, Niyogi, KK (2001) Non-photochemical quenching.A response to excess light energy. Plant Physiol 125:1558-1566.

  9. Krause GH,Weis E (1991) Chlorophyll fluorescence and photosynthesis: The basics.Annu Rev Plant Physiol Plant Mol Biol 42:313-349.




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C?MO CITAR (Vancouver)

Rev Educ Bioquimica. 2008;27