Leyva HER, Vega ME, Gómez RC, Díaz SI, Gutiérrez NP

Language: Spanish
References: 19
Page: 42-48
PDF size: 124.66 Kb.

ABSTRACT

Langerhans cells are generally located in squamous layer and seldom in lamina propia; they can be distinguished by their morphology and location. The aim of the present study was to assess their immunoexpression and distribution using S-100 protein in lichen planus and in pemphigus vulgaris. Twenty cases of lichen planus and 10 cases of pemphigus vulgaris were stained with S-100 (Protein Monoclonal, mouse IgG). The total average of S-100 positive cells in lichen planus was 5.15 ± 1.79 cells in epithelium and 2.82 ± 6.05 in lamina propia. Spearman correlation was applied obtaining rho = 1.02 (p £ 0.05). The average of cells in epithelium in pemphigus was 1.76 ± 1.43 and 1.78 ± 2.41 in lamina propia, rho = 0.8636 (p ≤ 0.025). Lichen planus showed a greater number of S-100 positive cells than pemphigus, lesions behaved in an inverse way, the expression in lichen planus was severe in epithelium and in pemphigus the expression was severe in lamina propia; this can be due to epithelium acantholysis in pemphigus.

REFERENCES

1. Di Franco C, Toto DP, Rowden G, Gargiulo WA, Keene JJ et al. Identification of Langerhans cells in human gingival epithelium. J Periodontol 1985; 56: 1; 48-54.

2. Manara GC, Pasquinelli G, Badiali-De Giorgi L, Ferrari C, Garatti SA, Fasanos D et al. Human epidermal Langerhans cells immunohistochemical and immunoelectron microscopical demonstration. Br J Dermatol 1996; 134: 22-27.

3. Taweechaisupapong S, Angsubhakron S et al. A comparative study of human epidermal Langerhans cell densities in 10 anatomical sites in healthy and disease skin. Br J Dermatol 1996; 134: 187-188.

4. Roitt I. Inmunología. Fundamentos. 4a ed. Madrid, España: Editorial Hartcourt Brace, 1997.

5. Ten C. Histología oral. Desarrollo, estructura y función. 2a ed. Buenos Aires, Argentina: Panamericana, 1986.

6. Ross M. Histología. 3a ed. México: Panamericana, 1997.

7. Barrett AW, Scully C. S-100 protein in oral biology and pathology. J Oral Pathol Med 1994; 23: 433-40.

8. Trwue D, Lawrence. Atlas of diagnostic immunohistopathology. New York: JB Lippincot, 1990.

9. Proksch E, Brasch J, Sterry W. Integrity of the permeability barrier regulates epidermal Langerhans cell density. Br J Dermatol 1996; 134: 630-638.

10. Ingafou M, Lodi G et al. Oral lichen planus is not associated with IgG circulating antibodies to epithelial antigens. Oral Surg, Oral Med, Oral Pathol, Oral Radiol Endod 1997; 84: 175-178.

11. Eversole LR. Immunopathology of oral mucosal ulcerative, desquamative and bullous diseases. Oral Surg, Oral Med and Oral Pathol 1994; 77: 555-571.

12. Walton Lj, Thornhill MH, Farthing PM. VCAM-1 and ICAM-1 are expressed by Langerhans cells, macrophages and endothelial cells in oral lichen planus. J Oral Pathol Med 1994; 23: 262-8.

13. Hietanen J, Paasonen MR, Kuhlefelt M, Malstrom M. A retrospective study of oral lichen planus patients with concurrent of subsequent development of malignancy. Oral Oncol 1999; 35(3): 278-282.

14. Karagouni EE, Dotsika EN, Sklavounou A. Alteration in peripheral blood mononuclear cell function and serum cytokines in oral lichen planus. J Oral Pathol Med 1994; 23: 28-35.

15. Mignogna MD, Lo Muzio L, Lo Russo L. Oral lichen planus: Different clinical features in HCV-positive and HCV-negative patients. Int J Dermatol 2000; 39: 134-139.

16. Beer K, Albertini J, Soltani K, Medenica M. Corps ronds In: oral pemphigus vulgaris. Int J Dermatol 1994; 33(5): 190-191.

17. Sciubba JJ. Autoimmune aspects of pemphigus vulgaris and mucosal pemphigoid. Adv Dent Res 1996; 10 (1): 52-6.

18. Daniels TE, Chou L, Greenspan JS, Hauck WW, Greene JC, et al. Reduction of Langerhans cells in smokeless tobacco-associated oral mucosal lesions. J Oral Pathol Med 1992; 21: 100-104.

19. Porter SR, Kirby Alun, Olsen I, Barret W. Immunologic aspects of dermal and oral lichen planus. Oral Surg, Oral Pathol, Oral Med Radiol, Endod 1997; 83: 358-66.