>Cirugía y Cirujanos
>Year 2013, Issue 1
Alfaro-Rodríguez A, González-Piña R, Bueno-Nava A, Ávila-Luna A, Arch-Tirado E
Measurement of acetylcholine and sleep patterns
Cir Cir 2013; 81 (1)
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Introduction: Acetylcholine is an essential neurotransmitter in the
central nervous system as it has an effect on sleep, memory and
learning. Intracerebral microdialysis is an in vivo surgical technique
that is used to measure the concentration of substances in the
Methods: The rats were stereotaxically implanted with
microdialysis cannulae guided to the hypothalamic medial preoptic
area and bipolar stainless steel electrodes to obtain sleep records
simultaneously with microdialysis samplings during 24 hours
exposed to clean air followed by 24 hours of exposure to ozone.
Microdialysis fractions were injected into chromatograph system.
Results: Results showed that paradoxical sleep and wakefulness
decreased by 54.2% and 27.9%, respectively, while slow wave
sleep increased by 35.1% during the ozone exposure phase and a
concomitant decrease of extracellular acetylcholine of 56.2% was
observed during the light–dark phase.
Conclusion: That surgical method employed using
electroencephalography and intracerebral microdialysis allows
the quantification of extracellular acetylcholine and simultaneously
with patterns related to sleep. We propose that the decrease in
paradoxical sleep is the behavioral expression of disruptions of
cholinergic modulation and, that post-exposure effects observed
in the hypothalamic medial preoptic area can be explained on the
basis of the hypothalamic role in the sleep–wake cycle.
||Acetylcholine, microdialysis, high-performance liquid chromatography, electroencephalography.
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>Cirugía y Cirujanos
>Year 2013, Issue 1