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>Journals >Cirugía y Cirujanos >Year 2013, Issue 1

Alfaro-Rodríguez A, González-Piña R, Bueno-Nava A, Ávila-Luna A, Arch-Tirado E
Measurement of acetylcholine and sleep patterns
Cir Cir 2013; 81 (1)

Language: Español
References: 38
Page: 21-27
PDF: 247.40 Kb.

[Full text - PDF]


Introduction: Acetylcholine is an essential neurotransmitter in the central nervous system as it has an effect on sleep, memory and learning. Intracerebral microdialysis is an in vivo surgical technique that is used to measure the concentration of substances in the extracellular space.
Methods: The rats were stereotaxically implanted with microdialysis cannulae guided to the hypothalamic medial preoptic area and bipolar stainless steel electrodes to obtain sleep records simultaneously with microdialysis samplings during 24 hours exposed to clean air followed by 24 hours of exposure to ozone. Microdialysis fractions were injected into chromatograph system.
Results: Results showed that paradoxical sleep and wakefulness decreased by 54.2% and 27.9%, respectively, while slow wave sleep increased by 35.1% during the ozone exposure phase and a concomitant decrease of extracellular acetylcholine of 56.2% was observed during the light–dark phase.
Conclusion: That surgical method employed using electroencephalography and intracerebral microdialysis allows the quantification of extracellular acetylcholine and simultaneously with patterns related to sleep. We propose that the decrease in paradoxical sleep is the behavioral expression of disruptions of cholinergic modulation and, that post-exposure effects observed in the hypothalamic medial preoptic area can be explained on the basis of the hypothalamic role in the sleep–wake cycle.

Key words: Acetylcholine, microdialysis, high-performance liquid chromatography, electroencephalography.


  1. Yamuy J, Fung SJ, Xi M, Morales FR, Chase MH. Hypoglossal motoneurons are postsynaptically inhibited during carbacholinduced rapid eye movement sleep. Neuroscience 1999;94(1):11-15.

  2. Gompf HS, Mathai C, Fuller PM, Wood DA, Pedersen NP, Saper CB, et al. Locus Ceruleus and Anterior Cingulate Cortex Sustain Wakefulness in a Novel Environment. J Neurosci 2010;30(43):14543-14551.

  3. Lu J, Greco MA, Shiromani P, Saper CB. Effect of Lesions of the Ventrolateral Preoptic Nucleus on NREM and REM Sleep. J Neurosci 2000;20(10):3830-3842.

  4. Imeri L, Opp MR. How (and why) the immune system makes us sleep. Nat Rev Neurosci 2009;10(3):199-210.

  5. Wilson S, Argyropoulos S. Antidepressants and Sleep: A Qualitative Review of the Literature. Drugs 2005;65(7):927-947.

  6. Marrosu F, Portas C, Mascia MS, Casu MA, Fà M, Giagheddu M, et al. Microdialysis measurement of cortical and hippocampal acetylcholine release during sleep-wake cycle in freely moving cats. Brain Res 1995;671(2):329-332.

  7. Prokai L, Zharikova AD. Neuropharmacodynamic evaluation of the centrally active thyrotropin-releasing hormone analogue [Leu2] TRH and its chemical brain-targeting system. Brain Res 2002;952(2):268-274.

  8. Zhu Y, Wong PS, Cregor M, Gitzen JF, Coury LA, Kissinger PT. In vivo microdialysis and reverse phase ion pair liquid chromatography/tandem mass spectrometry for the determination and identification of acetylcholine and related compounds in rat brain. Rapid Commun Mass Spectrom 2000;14(18):1695-1700.

  9. Semba K, Fibiger HC. Afferent connections of the laterodorsal and the pedunculopontine tegmental nuclei in the rat: a retro- and anterograde transport and inmunohistochemical study. J Comp Neurol 1992;323(3):387-410.

  10. Steriade M, McCarley RW. Brain Control of Wakefulness and Sleep. New York: Plenum, 1990 p. 326-353.

  11. Jones BE. Paradoxical sleep and its chemical/structural substrates in the brain. Neuroscience 1991;40(3):637-656.

  12. Szymusiak R, McGinty D. Control of slow wave sleep by thermoregulatory mechanisms. Prog Clin Biol Res 1990;345:53-64.

  13. Lin JS, Sakai K, Jouvet M. Hypothalamo-preoptic histaminergic projections in sleep-wake control in the cat. Eur J Neurosci 1994;6(4):618-625.

  14. Houdouin F, Cespuglio R, Jouvet M. Effects induced by electrical stimulation of the nucleus raphe dorsalis upon hypothalamic release of 5-hydroxyindole compounds and sleep parameters in the rat. Brain Res 1991;565(1):48-56.

  15. Kása P. The cholinergic systems in brain and spinal cord. Prog Neurobiol 1986;26:211-272.

  16. Asala SA, Okano Y, Honda K, Inoué S. Effects of medial preoptic area lesions on sleep and wakefulness in unrestrained rats. Neurosci Lett 1990;114(3):300-304.

  17. García-Arraras JE, Pappenheimer JR. Site of action of sleep-inducing muramyl peptide isolated from human urine: microinjection studies in rabbit brains. J Neurophysiol 1983;49(2):528-533.

  18. Talwar A, Kumar VM. Effect of carbachol injection in the medial preoptic area on sleep-wakefulness and body temperature in free moving rats. Indian J Physiol Pharmacol 1994;38(3):163-168.

  19. Gross KB, White HJ, Sargent NE. The effect of ozone inhalation on metabolic functioning of vascular endothelium and on ventilatory function. Toxicol Appl Pharmacol 1991;109(2):336-351.

  20. Onoe H, Watanabe Y, Ono K, Koyama Y, Hayaishi O. Prostaglandin E2 exerts an awaking effect in the posterior hypothalamus at a site distinct from that mediating its febrile action in the anterior hypothalamus. J Neurosci 1992;12(7):2715-2725.

  21. Imeri L, Bianchi S, Angeli P, Mancia M. Stimulation of cholinergic receptors in the medial preoptic area affects sleep and cortical temperature. Am J Physiol 1995;269(2):R294-R299.

  22. Velazquez-Moctezuma J, Gillin JC, Shiromani PJ. Effect of specific M1, M2 muscarinic receptor agonists on REM sleep generation. Brain Res 1989;503(1):128-131.

  23. Imeri L, Bianchi S, Angeli P, Mancia M. Muscarinic receptor subtypes in the medial preoptic area and sleep-wake cycles. Neuroreport 1996;7(2):417-420.

  24. Inouye ST. Does the ventromedial hypothalamic nucleus contain a self-sustained circadian oscillattor associated with periodic feedings? Brain Res 1983;279(1-2):53-63.

  25. Pittendrigh CS. Temporal Organization: Reflections of Dawinian Clock-Watcher. Annu Rev Physiol 1993;55:17-54.

  26. Ruzak B, Groos G. Suprachiasmatic stimulation phase shifts rodent circadian rhythms. Science 1982;215(4538):1407-1409.

  27. Ruzak B, Zucker I. Neural regulation of circadian rhythms. Physiol Rev 1979;59(3):449-526.

  28. Damsma G, WesterinK BHC. A microdialysis and automated online analysis aproach to study central cholinergic tramsmission “in vivo”. En: Robinson TE, Justice J, editores. Microdialysis in the neurosciences. Amsterdan: Elsevier, 1991 p. 237-252.

  29. Paxinos G, Watson C. The Rat Brain in Stereotaxic Coordinates. 4 th ed. New York: Academic Press, 1998 p. 84-121.

  30. Williams JA, Comisarow J, Day J, Fibiger HC, Reiner PB. Statedependent release of acetylcholine in rat thalamus measured by in vivo microdialysis. J Neurosci 1994;14(9):5236-5242.

  31. Klüver H, Barrera E. A Method for the Combined Staining of Cells and Fiber in the Nervous System. J Neuropathol Exp Neurol 1953;12(4):400-403.

  32. España RA, Scammell TE. Sleep neurobiology from a clinical perspective. Sleep 2011;34(7):845-858.

  33. Hackney JD, Linn WS, Karuza SK, Buckley RD, Pedersen EE, Law DC, et al. Effects of ozone exposure in Canadians and Southern Californians. Arch Environ Health 1977;32(3):110-116.

  34. Huitrón-Reséndiz S, Custodio-Ramírez V, Escalante-Membrillo C, González-Piña R, Paz C. Sleep alterations and brain regional changes of serotonin and its metabolite in rats exposed to ozone. Neurosc Lett 1994;177(1-2):119-122.

  35. Zetterström T, Sharp T, Marsden CA, Ungerstedt U. In Vivo Measurement of Dopamine and Its Metabolites by intracerebral Dialysis: Changes After d-Amphetamine. J Neurochem 1983;41(6):1769-1773.

  36. Marsden CA, Macdonald IA, Joseph MH, Perrett D. Electrochemical detection, HPLC and in vivo monitoring in the biosciences. J Neurosci Methods 1990;34(1-3):1-2.

  37. Dykstra KH, Hsiao JK, Morrison PF, Bungay PM, MefFord IN, Scully MM, et al. Quantitative Examination of Tissue Concentration Profiles Associated with Microdialysis. J Neurochem 1992;58(3):931-940.

  38. Benveniste H. Brain microdialysis. J Neurochem 1989;52:1667-1679.

>Journals >Cirugía y Cirujanos >Year 2013, Issue 1

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