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Patología Revista Latinoamericana

2013, Number 1

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Patol Rev Latinoam 2013; 51 (1)

Autopsy by selective sampling with cutting needle. Diagnostic utility in a case with HIV infection and disseminated histoplasmosis

Salazar-Morales MF, Parraguirre-Martínez S, Pérez-Torres A, Sánchez-Cervantes IG

Language: Spanish
References: 22
Page: 18-22
PDF size: 359.12 Kb.


ABSTRACT

A case of HIV infection with minimally invasive autopsy through needle biopsy is reported.
Case. 36 year old man referred with HIV diagnosis. He lost consciousness and was brought to emergency department, then hospitalized after receiving medical evaluation but died the next day. Autopsy was authorized.
Procedure. Percutaneous samples were taken from five different sites corresponding to the three right pulmonary lobes (superior, middle and low) and the two left pulmonary lobes (superior and low) using a semiautomatic 14G and 15.2 cm soft tissue biopsy needle.
Findings. Histological examination showed lung tissue except in two samples where liver and spleen tissue were identified. A granulomatous reaction was observed with histiocytes containing yeast forms compatible with Histoplasma capsulatum. Immunohistochemical detection confirmed the yeasts identity as Histoplasma capsulatum var. capsulatum.
Conclusion. Needle necropsy is an easy, quick and useful procedure for postmortem diagnosis in cases where an histopathological alteration needs to be defined and conventional necropsy represents a dangerous procedure for prosector´s safety or when family´s concerns do not allow the complete postmortem exam.


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