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ISSN 2007-6509 (Print)
Órgano oficial de la Asociación Mexicana de Medicina Transfusional A.C.

2013, Number 1

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Rev Mex Med Transfus 2013; 6 (1)

An suitable harvest of total nucleated cells from umbilical cord blood

Benítez-Arvizu G, Ibarra-Pichardo Y, Guerra-Márquez Á, Malagón-Martínez A

Language: Spanish
References: 14
Page: 7-11
PDF size: 179.32 Kb.


ABSTRACT

Introduction: To execute hematopoyetic stem cell transplantation is necessary calculated a cellular dose measuring the nuclead total cells (NTC) per kilogram, to more cellularity is cover a mayor kilograms. Identify variable while the recollection of cord blood makes reach a optimal CNT count. Material and methods: Units from Cord Blood Bank from Centro Médico Nacional «La Raza» in Mexico City, from February 2006 to February 2008, contemplated: initial volume unit, gesta, weeks of gestation, NTC. Assessment the correlation using a student t and Kruskal Wallis statistics in each case, following a ROC construction to delimit the cut off for the volume considered a 8 x 108 NTC as minimum in significative variables. Results: Were analyze 250 cord blood units (BCU); 92.75 mL as initial volume mean, 6.63 x 108 TNC as mean, 39 gestation weeks as mode and one gesta as mode. The TNC correlated with the initial volume (Student´s t, p ‹ 0.001). The cut off to reach 8 x 108 TNC is 75 mL with 89% of sensibility. Conclusion: The initial volume shows sadistic correlation to achieve an adequate TNC harvest.


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