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Órgano oficial de difusión de la Federación Mexicana de Patología Clínica, AC y de la Asociación Latinoamericana de Patología Clínica/Medicina de Laboratorio

2014, Number 3

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Rev Mex Patol Clin Med Lab 2014; 61 (3)

Determination of cut-off values in healthy donors for lupus anticoagulant detection protocol in the Laboratorio Médico Echavarría, according to the international guidelines

Saldarriaga-Saldarriaga M, Restrepo-Lozada MA, Montoya-López GP

Language: English
References: 12
Page: 145-149
PDF size: 196.98 Kb.


ABSTRACT

Background: The main types of antiphospholipid antibodies are: lupus anticoagulant, anticardiolipin antibodies and anti-b2 glycoprotein 1. Lupus anticoagulant has been associated with clinical findings such as thrombosis, consecutive fetal losses, and thrombocytopenia. Variables can affect the interpretation of the results. Objective: To determine the cut-off values in healthy donors for the group of tests required in detecting lupus anticoagulant, in order to improve the specificity of the test. Methods: Thirty-six healthy donors, from whom the cut-off values were calculated. The parameters measured in the study were: baseline activated partial thromboplastin time, aPTT 1:1 mix with normal plasma pool, and screen and confirmatory dilute Rusell viper venom time (dRVVTs/ dRVVTc). Results: The cut-off values obtained from percentage correction, Index of Circulating Anticoagulant, dRVVTs ratio, dRVVTc ratio, and final ratio for Laboratorio Médico Echavarría were: 27.6%, 12.18, 1.19, 1.11 and 1.24, respectively. Conclusions: Regarding the means of dRVVTs and dRVVTc ratios, the results were very similar to those obtained in other studies. The cut-off value obtained for the ICA and the correction rate were 12.2 and 27.6%, respectively, while the cut-off value reported in the literature is 15 and 10% for both tests, respectively.


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