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Órgano Ofical de la Facultad de Estomatología de la Benemérita Universidad Autónoma de Puebla

2014, Number 47

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Oral 2014; 15 (47)

In- Vitre mercury Cytotoxicity on polymorph nuclear leukocytes

Soto GME, Tinaco CVC, lsassi HH, Padilla CJ, Trejo TSE, Oliver PR, Huitzil MEE

Language: Spanish
References: 32
Page: 1070-1073
PDF size: 380.72 Kb.


ABSTRACT

Abstract. Dental amalgam has been usually used as the selectiva material in dentals restorations, alloy consisting mainly of silver, tin, copper and zinc materials wich are found mixed with the Mercury. Mercury is a chemical element (metal) naturally in the environment ond the people could be exposed to mercury in their diet, water, pollution ond dental amalgams. People with amolgom fillings are chronically exposed to low doses of mercury. In this poparon interesting material to research is presentad looking potentially effed that mercury could do on the neutrophil polymorphonuclear leukocytes. Objedive. The oim of this study was to evaluote the ln-Vitro Cytotoxicity from HgCI2 (mercury chloride) on LPMN. Materials ond methods. 1 Oml of blood somple was token from dinical healthy volunteer donors and their neutrophil orcl􀀝 polymorphonuclear leukocytes were seporated and the population wos adjusted and challenged with HgCI2 solution 1 OmM for one hour ond 2 hours keeping the following concentrations: 2 µg/ml, 4 µg/ml, 6 µg/ml, 12 ug/ml. Results. The results were obtained using the SPSS version 18.0 Statistic were that at concentrations of 12ug /mi the mercury chloride affected the viability of neutrophi! polymorphonuclear leukocytes following a deseresponse pattern where concentration (l2 µg/ml HgCI2) affeded the viability of neutrophil polymorphonuclear leukocyte. Conclusions. The results showed a response the higher concentration (l2 µg/ml HgCI2) lower viability.


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