2002, Number 1
Vet Mex 2002; 33 (1)
Evaluation of Eimeria tenella oocyst total count carried out with both the McMaster camera and the Neubauer haemocytometer from faeces or caecal tissue samples
Juárez EMA, Cabriales JJJ, Petrone GVM, Téllez IG
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ABSTRACTResearch evaluation regarding Eimeria spp requires a trustworthy oocyst count technique. The aim of this study was to determine the efficacy of total oocyst count using the Neubauer haemocytometer and the McMaster camera. E. tenella sporulate oocysts were inoculated per os to two groups of Leghorn chicks. Faecal samples were collected at seven days postinoculation, and different technicians counted oocysts in both cameras. The Hartley test was used to analyze homocedasticity variance level within counts with each camera. One counting, set by the same technician, showed variance heterocedasticity in one from two counting samples with the haemocytometer but not with the McMaster camera. Furthermore, there was a significant difference in the total oocyst counts carried out with the McMaster camera between two technicians but not with the Neubauer haemocytometer. It is concluded that the Hartley test is a trustworthy statistical method to evaluate laboratory techniques.