Nodarse HR, Fuerte CME

Language: Spanish
References: 15
Page: 33-40
PDF size: 104.10 Kb.

ABSTRACT

Introduction: currently, worldwide, the most serious hospital infections include Acinetobacter strains resistant to multiple antibiotics.
Objective: phenotypically identify Acinetobacter spp and characterize their resistance to antibiotics.
Methods: an observational study was conducted in a sample of 60 Acinetobacter spp strains of hospital waste origin, which were isolated in "Dr. Luis Díaz Soto" central military hospital in 2014 first quarter. These strains were initially classified according to Bouvet and Grimont scheme (modified by Gemer-Smidt and others). Subsequently these strains underwent antimicrobial susceptibility testing with disks: piperacillin, piperacillin/tazobactam, cefoperazone, ceftriaxone, ceftazidime, cefepime, imipenem, meropenem, aztreonam, gentamicin, amikacin and ciprofloxacin; E-test: colimycin and finally, in vitro biofilm production by slime-test technique.
Results: the implementation of this scheme only allowed to phenotypically identify Acinetobacter baumannii strains (88.3 %); in other cases the identification was not conclusive. 51.6 % of Acinetobacter baumannii strains was isolated in the adult intensive care unit and 46.6 % from bronchial secretions. Acinetobacter baumannii resistance was 80 % higher, which contrasted with the no-baumannii strains low resistance. The e-test implementation for colimycin in Acinetobacter baumannii strains found 90 % inhibited at minimum inhibitory concentrations ≤ 0.5 µg/mL. All Acinetobacter baumannii strains were biofilm producing.
Conclusions: Acinetobacter baumannii strain is one of the most isolated microorganisms in critical patients in hospital, with significant bacterial resistance, except for colimycin.

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