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2013, Number 4

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Rev Cubana Invest Bioméd 2013; 32 (4)

Design of a medium based on vegetable peptones for the culture of Clostridium chauvoei

Arias-Palacios J, Isaza-Pérez A, Martínez-Acosta D

Language: Spanish
References: 13
Page: 445-456
PDF size: 160.23 Kb.


ABSTRACT

Objective: a culture medium based on vegetable peptones was designed for the production of Clostridium chauvoei biomass.
Methods: nine vegetable peptones and two vegetable peptone broths were selected to substitute animal peptone in the modified Clostridium medium. A fermentation process was developed which was related to the production of cell mass, and absorbance and transmittance percentage (%T) were verified. The second stage included pathogenicity assays to determine LD50 titers.
Results: vegetable peptones 1-B, 8-M, 10-M, 11-M were selected, for their % T was close to that of the modified Clostridium medium. A lethality test (LD50) was then performed on guinea pigs. Cultures with these vegetable peptones presented LD50 between 10-8 and 10-9 dilutions, in contrast to the 106.5 obtained in cultures with the control medium.
Conclusions: vegetable peptones 1-B, 8-M, 10-M and 11-M stimulated the production of Clostridium chauvoei biomass at pH 8.2. The vegetable peptones tested showed greater lethality than the base medium.


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