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2015, Number 3

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Rev Hematol Mex 2015; 16 (3)

A new protocol for obtaining platelet rich in growth factors (PRP). A descriptive study in 15 patients and comparison with literature

Alcaraz-Rubio J, Oliver-Iguacel A, Sánchez-López JM
Full text How to cite this article

Language: Spanish
References: 6
Page: 210-216
PDF size: 643.73 Kb.


Key words:

platelet-rich plasma, growth factors, centrifugation, open technique, laminar flow bell.

ABSTRACT

Background: The use of platelet growth factors, or what is commonly known as platelet-rich plasma (PRP), is becoming a technique considered medication, despite sharing features of the autotransfusion, with multiple clinical applications in different fields of Medicine.
Objective: To introduce and test a specific technique for obtaining PRP, with precise characteristics both production and final composition of compound got, in 15 hematological healthy patients, comparing our results with those obtained by other procedures scientifically tested.
Material and method: A descriptive, series of clinical cases study that selected 15 Caucasian patients, 8 male, with ages from 5 to 65 years, hematologically healthy, following the analytical norms of inclusion for autotransfusion of Spanish Society of Hematology and Hemotherapy about biochemical, hematological and serological controls previous to obtain samples of total blood.
Results: The protocol obtained levels of platelets and leukocytes about three and five times higher than basal data with predominance of mononuclear (75-90% of white cell).
Conclusions: The procedure proposed obtains a higher quantity of platelets compared to other protocols of treatment consulted, even though it is not related to quantity of growth factor obtained independently of age and sex of patient.


REFERENCES

  1. Beca T, Hernández G, Morante S, Bascones A. Plasma rico en plaquetas. Revisión bibliográfica. Av Periodon Implantol 2007;19:39-52.

  2. Alsousou J, Thonpson M, Hulleyp, Noble A, Willett K. The biology of platelet-rich plasma and its applications in trauma and orthopaedic surgery. A review of literature. J Bone Joint Surg Br 2009;91-B:987-996.

  3. Anitua E, Prado R, Sánchez M, Orive G. Platelet-rich plasma: preparation and formulation. Oper Tech Orthop 2012;22:25-32.

  4. Weibrich G, Kleiss WKG, Hafner G, Hitzler WE. Growth factors level in platelets-rich plasma and correlations with donor age, sex and platelets count. J Craneomaxillofac Surg 2002;30:97-102.

  5. Everts PA, Brown-Mahoney C, Hoffmann JJ, Schonberger JP, et al. Platelet-rich plasma preparation using three devices: implications for platelets activation and platelet growth factors release. Growth Factors 2006;24:165-171.

  6. González-Villalva A. Capítulo 6. Sangre. En: Fortoul y Castell. Histología y Biología celular. México: McGraw-Hill Interamericana, 2010;147-154.




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Rev Hematol Mex. 2015;16