Becerril PDE, Torres GE, Moreno CG, Aguilar SAS, Arenas GR, Hernández CR

Language: Spanish
References: 13
Page: 214-219
PDF size: 179.96 Kb.

ABSTRACT

PCR (polymerase chain reaction) has become one of the most commonly employed techniques in molecular biology. Its function is to copy millions of specific targets in the DNA sequence through denaturation, primer annealing and extension. In dermatology and mycology PCR has been used for identification of many infectious agents such as Mycobacterium tuberculosis, atypical mycobacteria, M. leprae, Bartonella henselae; spirochetes Treponema pallidum and Borrelia burgdorferi; Herpes simplex-1 and 2, Varicella zoster virus, Human herpesvirus-8, Epstein-Barr virus; parasites such as Leishmania spp. Fungus as Aspergillus spp., Blastomyces dermatitidis, Candida spp., Coccidioides immitis, Cryptococcus neoformans, Histoplasmosis capsulatum and Paracoccidioidomycosis brasiliensis, among others. Also, PCR is used to analyze and search clonality of lymphocytes in some cutaneous lymphomas, and its relationship with different viruses, such as Epstein-Barr virus in cutaneous T-Cell and B-Cell lymphomas as well as NK nasal Lymphoma. In genodermatosis it has proved to be an excellent tool, useful in showing X-linked ichthyosis, and junctunial epidermolysis bullosa, among others. Also, in primary melanoma it is been used for detection of BRAF gene mutation, as well as melanocytic nevi.

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