Rodea-Rubio ME, Mendoza-Portillo E, Ruiz-González P, Lozano-Nuevo JJ, Suárez-Cuenca JA, Flores-Alcántar MG

Language: Spanish
References: 5
Page: 523-527
PDF size: 472.02 Kb.

ABSTRACT

Background: Infection by influenza virus requires better and more affordable diagnostic tests to improve the identification and timely therapy.
Objective: To compare the diagnostic performance of different tests for influenza.
Material and method: A cross-sectional, analytic, observational study was done of evaluation of diagnostic tests in comparison with test PCR-INDRE, considered the gold standard, in which 419 records of patients attending to General Hospital Ticomán from 2009 to 2014, diagnosed with influenza and severe acute pneumonia were analyzed. Time to get the results of each test was compared, as well as the diagnostic ability of polymerase chain reaction test from the National Institute of Epidemiological Diagnosis and Reference (PCR-INDRE), considered the gold standard, the National Polytechnic Institute test (IPN-PCR) and the Institutional Rapid test (PrP-SSDF).
Results: Average time for reporting: PCR-INDRE, 10 days; PCR-IPN, 3 days and PrP-SSDF, immediately. Regarding the gold standard, the sensitivity, specificity, positive predictive value and negative predictive value for PCR-IPN vs PrP-SSDF were: 0.95 vs 0.43; 0.90 vs 0.90; 0.80 vs 0.87 and 0.98 vs 0.85, respectively.
Conclusions: Comparison of PCR-IPN and PrP-SSDF showed that the former had the best diagnostic performance for influenza and the availability in time was reasonable. It is proposed as an affordable and valuable diagnostic choice.

REFERENCES

1. Noyola DE, Clark B, O'Donnell FT, Atmar RL, et al. Comparison of a new neuraminidase detection assay with an enzyme immunoassay, immunofluorescence, and culture for rapid detection of influenza A and B viruses in nasal wash specimens. J Clin Microbiol 2000;38:1161-1165.

2. Secretaría de Salud (SSA). Plan Nacional de Preparación y Respuesta ante una Pandemia de Influenza. México, 2006.

3. Miller MA, Viboud C, Balinska M, Simons L. The signature features of influenza pandemics – Implications for policy. N Engl J Med 2009;360:2595.

4. Tothberg MB, Haessler SD, Brown RB. Complications of viral influenza. Am J Med 2008;121(4):258-64.

5. Fouchier RAM, Bestebroer TM, Herfst S, Van Der Kemp L, et al. Detection of influenza A viruses from different species by PCR amplification of conserved sequences in the matrix gene. J Clin Microbiol 2000;38:4096-4101.