Salazar EEL, Calzada SL

Language: Spanish
References: 16
Page: 128-131
PDF size: 66.58 Kb.

ABSTRACT

Background: -Na⁺-K⁺ ATPase is a useful marker which determines the origin of breast cancer cells.
Objective: -Na⁺-K⁺ ATPase activity, as well as viability of plasma membrane vesicles isolated from breast carcinoma tissues were demonstrated by histochemical detection.
Patients and method: Breast carcinoma tissue samples of patients who attended consultation in the oncology service at Hospital de Ginecoobstetricia Núm. 4 Dr. Luis Castelazo Ayala, IMSS were examined. Tissue samples from adenocarcinoma were homogenized in 4 volumes of TED solution at 4°C (Tris-HCl 0.01 M, EDTA 0.0015 M, ditiotreitol 0.001 M, pH 7.4) and subsequently centrifuged. The collected sample was homogenized and stratified in a discontinuous sucrose gradient (20 to 50%) and then centrifuged for 60 min at 30,000 xg. In order to determine -Na⁺-K⁺ ATPase activity in plasma membrane vesicles, suspension was incubated at Tris-maleate 0.04 M, adenosine triphosphate (ATP) 0.004 M, Mgcl 20.004 M, NaCl 0.1 M y Pb (NO₃) 20.005 M, pH 7.0. Reactions were carried out for 15 min at 37°C in prefixed vesicles in 3% glutaraldehyde in 0.1 M, cacodylate buffer stock, pH7.4 for 60 min.
Results: Histochemical detection demonstrated membrane vesicles from breast carcinoma tissues and proved their viability after tumoral progression.

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