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ISSN 1561-3054 (Electronic)

2015, Number 2

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Rev Cubana Med Trop 2015; 67 (2)

Detection of Brucella spp using a commercial system in enviroment cuban samples

Obregón FAM, Cabrera AA, Echevarría PE, Rodríguez OY, Rodríguez SJ

Language: Spanish
References: 12
Page: 183-192
PDF size: 108.11 Kb.


ABSTRACT

Introduction: Information about the circulation of Brucella species in the Cuban environment is not currently available due to the lack of laboratory methods for their identification.
Objectives: Detect Brucella spp. in Cuban environmental samples using a commercial immunochromatographic system.
Methods: A study was conducted of 59 environmental samples from an endemic zone for bovine brucellosis and 50 environmental samples from areas where the disease has been controlled, from December 2011 to February 2012. Use was made of the direct lateral flow immunochromatographic system for Brucella spp. commercially available in Cuba.
Results: Of the environmental samples from the endemic zone 52.5% (31/59) tested positive for Brucella spp. The environmental samples showing the highest positivity were those taken from dung (62.5%) and cemented soil (26.9%). Strongly positive reactions predominated in 74.1% (23/31) of the samples.
Conclusions: The commercial immunochromatographic system used detected a high percentage of Brucella spp. in environmental samples from the endemic zone, which could justify its implementation in the Public Health laboratory network and the Institute of Veterinary Medicine of Cuba. The results obtained should be complemented with Brucella isolates from the environment, human patients and animals.


REFERENCES

  1. Eales, K. M., R. E. Norton y N. Ketheesan: Brucellosis in northem Australia. Am J Trop Med Hyg. 2010;83(4):876-8.

  2. World Health Organization: Brucellosis in humans and animals. 2012;WHO/CDS/EPR USA.

  3. Scholz HC, Hubalek Z, Nesvadbova J, Tomaso H, Vergnaud G, P Le Flèche AM, et al. Isolation of Brucella microti from soil. Emerg Infect Dis. 2008;14(8):1316-7.

  4. Don J Brenner, Noel R Krieg, James T Staley, George M Garrity. The Proteobacteria. En: Bergey’s Manual on Systematic Bacteriology. Second Edition. Vol 2.USA. 2005. p. 370-85.

  5. Jiménez de Bagüés MP, Ouahrani - Bettache S, Quintana JF, Mitjana O, Hanna N, Bessoles S, et al. The new species Brucella m icroti replicates in macrophages and causes death in murine models of infection. J Infect Dis. 2010;202(1):3-10.

  6. Ariza J. Brucelosis en el siglo XXI. Med Clin (Barc). 2002;119(9):339-41.

  7. Araj GF. Update on laboratory diagnosis of human brucellosis. International Journal of Antimicrobial Agents. 2010;36:12-17.

  8. SD Standard Diagnostics, Inc. 2012. [Actualizado en 2012]. [Consultado: Enero 2015]. Disponible en: http://www.standardia.com.

  9. Instituto de Medicina Veterinaria (IMV). La Habana: Ministerio de la Agricultura (MINAGRI);2012.

  10. Earhart K, Vafakolov S, Yarmohamedova N, Michael A, Tjaden J., Soliman A. Risk factors for brucellosis in Samarqan - Oblast, Usbekistan. Int J Infect Dis. 2009;13(6):749-53.

  11. Aune K, Rhyan JC, Russell R, Roffe TJ, Corso B. Environmental persistence of Brucella abortus in the Greater Yellowstone Area. The Journal of Wildlife Management. 2012;76(2):253-61.

  12. Alexander KA, Blackburn JK, Vandewalle ME, Pesapane R, Baipoledi EK, Elzer PH (2012) Buffalo, Bush Meat, and the Zoonotic Threat of Brucellosis in Botswana. PLoS ONE 7(3):e32842. doi:10.1371/journal.pone.0032842




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