2015, Number 2
<< Back Next >>
Rev Cubana Med Trop 2015; 67 (2)
Evaluation of different samples for Mycobacterium leprae molecular detection in Cuba
Ruiz-Fuentes JL, Diaz-Garcia A, Suárez MO, Torres P, Acosta SL
Language: Spanish
References: 17
Page: 193-201
PDF size: 117.17 Kb.
ABSTRACT
Introduction: Currently, the early detection and treatment with multitherapy are the main strategy to control the infection by
Mycobacterium leprae. The objective of the present study was to evaluate the usefulness of different clinical samples for
molecular diagnosis of
M. leprae infection.
Methods: Twenty two patients with suggestive clinical leprosy were analyzed. Different clinical samples were taken by slit skin smear, histopathology and PCR. To determine the sensitivity and specificity of the PCR method, a case-control study was also performed using 40 slides from negative smears of patients with leprosy
paucibacillary and 40 from individuals without leprosy.
Results: From all patient studied fifty-four percent were positive by slit skin smear and 41% were conclusive of leprosy by histopathology.
M. leprae DNA was detected in slit skin smears and lymph swabs in 100% of patients. In 95,45% of patients were detected
M. leprae DNA in whole blood and in 31,8% of them in nasal swab. The sensitivity of PCR respect to conventional diagnostic was 100%,t he specificity was 97.5%, and the positive predictive value was 97.56 and the negative predictive value was100%.
Conclusion: The diagnosis of
M. leprae by PCR is very useful when conventional techniques are inconclusive. The slit skin smears and lymph swabs are the most useful clinical samples for molecular confirmation of infection with
M. leprae.
REFERENCES
OMS: Weekly Epidemiological Record, Global Leprosy situation. 2012;34.
Kamble R, Shinde V, Madhale S, Kamble A, Ravikumar B, Jadhav R. Extraction and detection of Mycobacterium leprae DNA from ZNCF-stained skin smear slides for better identification of negative skin smears. Indian Journal of Medical Microbiology. 2010;28(1):57-9.
MINSAP. Programa de control de Lepra. 2 ed. La Habana: Editorial Ciencias Médicas;1999;1-2.
Donoghue H, Holton J, Spigelman M. PCR primers that can detect low levels of Mycobacterium leprae DNA. J Med Microbiol. 2001;50(2):177-82.
MINSAP. Lepra. Normas técnicas para el control y tratamiento. Editorial Ciencias Médicas 2008.
Ridley D. Histological classification and the immunological spectrum of leprosy. Bull World Health Organ. 1974;51(5):451-65.
Beyene D, Aseffa A, Harboe M, Kidane D, Macdonald M, Klatser P, et al. Nasal carriage of Mycobacterium leprae DNA in healthy individuals in Lega Robi village, Ethiopia. Epidemiol Infect. 2003 131(2):841-8.
Cardona-Castro N, Beltrán-Alzate J, Manrique-Hernández R. Survey to identify Mycobacterium leprae-infected household contacts of patients from prevalent regions of leprosy in Colombia. Mem Inst Oswaldo Cruz. 2008;103(4):332-6.
Torres P, Camarena J, Gomez J, Nogueira J, Gimeno V, Navarro J, et al. Comparison of PCR mediated amplification of DNA and the classical methods for detection of Mycobacterium leprae in different types of clinical samples in leprosy patients and contacts. Lepr Rev. 2003;74(1):18-30.
Patrocínio L, Goulart I, Goulart L, Patrocínio J, Ferreira F, Fleury R. Detection of Mycobacterium leprae in nasal mucosa biopsies by the polymerase chain reaction. FEMS Immunol Med Microbiol. 2005;44:311-6.
Martinez T, Figueira M, Costa A, Gonçalves M, Goulart L, Goulart I. Oral mucosa as a source of Mycobacterium leprae infection and transmission and implications of bacterial DNA detection and the immunological status. Clin Microbiol Infec. 2011;17:1653-8.
de Wit M, Douglas J, McFadden J, Klatser P. Polymerase chain reaction for detection of Mycobacterium leprae in nasal swab specimens. J Clin Microbiol. 1993;31(3):502-6.
de Almeida E, Nóbrega Martinez A, Câmara Maniero V, Sales A, Duppre N, Sarno E, et al. Detection of Mycobacterium leprae DNA by Polymerase Chain Reaction in the Blood and Nasal Secretion of Brazilian Household Contacts. Mem Inst Oswaldo Cruz. 2004;99(5):509-12.
Araújo S, Lobato J, de Melo Reis E, Bernardes Souza D, Gonçalves M, Vieira Costa A, et al. Unveiling healthy carriers and subclinical infections among household contacts of leprosy patients who play potential roles in the disease chain of transmission Mem Inst Oswaldo Cruz, Rio de Janeiro. 2012;107
Rao R, Mohan V, Shenoi S. Bacteremia in leprosy. Journal of Pakistan Association of Dermatologists. 2007;17:14-5.
Sigit Prakoeswa C. Detection of Mycobacterium leprae DNA in Blood of the Subclinical Leprosy. Media Folia Medica Indonesiana. 2007;43(2):64-7.
Wen Y, Xing Y, Yuan L, Liu J, Zhang Y, Li H. Whole-blood nested-PCR amplification of M. leprae-specific DNA for early diagnosis of leprosy. Am J Trop Med Hyg. 2013;88(5):918-22.
Full text