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2016, Number 2

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Rev Cubana Plant Med 2016; 21 (2)

In vitro effect of extract of Lotus corniculatus L. on bovine gastrointestinal nematodes

Rodríguez MCE, Reatiga YC, Gómez LDF

Language: Spanish
References: 22
Page: 145-156
PDF size: 126.82 Kb.


ABSTRACT

Introduction: The parasites are considered as one of the most important issues affecting cattle production due to the decline in productive and reproductive parameters.
Objective: Determine the effect of Lotus corniculatus L. extract on in vitro bovine gastrointestinal nematodes.
Methods: Leaves were used in the dry state with L. corniculatus regrowth age 45 to 70 days, and the ethanolic extract was obtained by leaching, the extract was stored at 4°C, the pure extract was used and dilutions obeying a range of 1.5mg/mL. To determine the effect of the extract worked through the technique of hatching eggs (Egg hatch assay) for nematodes: Bunostomum ssp, Trichostrongylus and Strongyloides papilosus, on the effective minimum percentage of 60%. The presence of tannins was determined.
Results: Inhibition of egg hatching S. papilus with maximum concentration and dilution 1 and 2, to extract 45 days of regrowth, with percentages of 100%, 86% and 66.5% respectively was found; extract regrowth 70 days showed a percent inhibition of hatching only the maximum concentration and dilution 1,98.5% and 78.5%. For T. colubriformis the only was ineffective dilution was 4 dilution extract regrowth 70 days with a percentage of 50.5% and for Bunostomun spp extract was effective with a high concentration and dilution 1 for extract 45 and 70 days of regrowth, with rates of 10 0% and 69.5% for 45 days and 100% and 68.5% for 70 days.
Conclusions: L. corniculatus extract 45 and 70 days of regrowth showed the most satisfactory results in inhibition of hatching eggs of T. colubriformis.


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