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ISSN 1029-3043 (Electronic)

2016, Number 4

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Medicentro 2016; 20 (4)

Identification of subpopulations according to sperm head morphometry in men with normal spermiogram results

Triana PI, López PR, García GMB, Pérez PPN, Menéndez HEM, Sánchez FP

Language: Spanish
References: 20
Page: 278-287
PDF size: 212.70 Kb.


ABSTRACT

Introduction: morphometry is a valuable tool to evaluate sperm morphology, as well as, it allows the identification of different subpopulations, which makes possible a better evaluation of spermatozoa morphology.
Objective: to identify existing sperm subpopulations in the studied population according to sperm head morphometric variables.
Methods: an analytic observational study of the morphometric characteristics of 1 200 spermatozoa from the seminal samples of 60 men with normal spermiogram results seen in the Assisted Reproduction Unit of Villa Clara province was carried out between January and March, 2014. Methods to analyze conglomerates were used to identify morphometric subpopulations.
Results: three subpopulations were identified in the studied population according to the sperm head morphometry.
Conclusions: only one of the three identified morphometric subpopulations had normal spermatozoa taking into consideration the morphologic and morphometric established criteria.


REFERENCES

  1. Rojas Quintana P, Medina Tío DM, Torres Ajá L. Infertilidad. Medisur [internet]. 2011 [citado 25 mayo 2015];9(4):[aprox. 11 p.]. Disponible en: http://medisur.sld.cu/index.php/medisur/article/view/1692/753

  2. Dohle GR, Diemer T, Giwercman A, Jungwirth A, Kopa Z, Krausz C. Guía clínica sobre la infertilidad masculina. European Association of Urology [internet]. 2010 [citado 25 mayo 2015]. Disponible en: http://www.aeu.es/UserFiles/files/13- GUIA%20CLINICA%20SOBRE%20INFERTILIDAD%20MASCULINA.pdf

  3. Menkveld R. Clinical significance of the low normal sperm morphology value as proposed in the fifth edition of the WHO laboratory manual for the examination and processing of human semen. Asian J Androl [internet]. 2010 Jan. [citado 31 mayo 2015];12(1):[aprox. 12 p.]. Disponible en: http://www.asiaandro.com/news/upload/20130621-aja200914a.pdf

  4. Menkveld R, Holleboom CA, Rhemrev JP. Measurement and significance of sperm morphology. Asian J Androl [internet]. 2010 Nov. 15 [citado 7 dic. 2015];13(1):[aprox. 6 p.]. Disponible en: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3739393/

  5. Menkveld R. Sperm morphology assessment using strict (Tygerberg) criteria. Methods Mol Biol [internet]. 2013 [citado 8 feb. 2015]; 927:[aprox. 11 p.]. Disponible en: http://www.ncbi.nlm.nih.gov/pubmed/22992902

  6. World Health Organization. WHO laboratory manual for the examination and processing of human semen. 5th. ed. [internet]. Geneva: World Health Organization Press; 2010 [citado 4 mayo 2015]. Disponible en: http://whqlibdoc.who.int/publications/2010/9789241547789_eng.pdf

  7. Gimeno Miquel IM. Morfología espermática y parámetros seminales básicos en varones normo y oligoastenoteratozoospérmicos [tesis]. Valencia, España: Universidad Politécnica de Valencia; 2014. [citado 20 Feb 20015]. Disponible en: http://riunet.upv.es/bitstream/handle/10251/46241/TFG%20Isabel%20Gimeno.pdf?sequence=1

  8. Maree L, du Plessis SS, Menkveld R, van der Horst G. Morphometric dimensions of the human sperm head depend on the staining method used. Hum Reprod [internet]. 2010 Apr. 17 [citado 9 mayo 2015];25(6):[aprox. 13 p.]. Disponible en: http://humrep.oxfordjournals.org/content/25/6/1369.full

  9. Mossman JA, Pearson JT, Moore HD, Pacey AA. Variation in mean human sperm length is linked with semen characteristics. Hum Reprod [internet]. 2012 Oct. 28 [citado 4 mar. 2015];28(1):[aprox.10 p.]. Disponible en: http://humrep.oxfordjournals.org/content/28/1/22.full

  10. Maroto Morales A. Evaluación objetiva de la morfometría de los espermatozoides de ovino (ovis aries). Relaciones con la fertilidad. [tesis]. Albacete, España: Universidad de Castilla-La Mancha; 2012. [citado 13 abr. 2015]. Disponible en: http://www.uclm.es/irec/Ecologia/pdf/repro_tesis/Tesis%20Doctoral%20Alejandro%20Maroto% 20Morales.pdf

  11. Buzón Cuevas A. Análisis cinético y morfométrico del espermatozoide del caballo empleando el sistema sperm class analyzer [tesis]. Córdoba, España: Universidad de Córdoba; 2014 [citado 4 abr. 2015]. Disponible en: http://helvia.uco.es/xmlui/bitstream/handle/10396/11586/2014000000902.pdf?sequence=1

  12. Armengol MF, Sabino GA, Forquera JC, de La Casa A, Aisen EG. Sperm head ellipticity as a heat stress indicator in Australian merino rams (ovis aries) in Northern Patagonia, Argentina. Theriogenology [internet]. 2014 Oct. 30 [citado 16 jun. 2015];83(4):[aprox. 7 p.]: Disponible en: http://www.ncbi.nlm.nih.gov/pubmed/25443418

  13. García-Peiró A, Oliver-Bonet M, Navarro J, Abad C, Amengual MJ, López-Fernández C, et al. Differential clustering of sperm subpopulations in infertile males with clinical varicocele and carriers of rearranged genomes. J Androl [internet]. 2012 May-Jun. [citado 9 oct. 2014];33(3):[aprox. 7 p.]. Disponible en: http://onlinelibrary.wiley.com/doi/10.2164/jandrol.111.013722/full

  14. Vicente-Fiel S, Palacín I, Santolaria P, Yániz JL. A comparative study of sperm morphometric subpopulations in cattle, goat, sheep and pigs using a computer-assisted fluorescence method (CASMA-F). Anim Reprod Sci [internet]. 2013 Apr. 12 [citado 23 mayo 2015];139(1-4):[aprox. 8 p.]: Disponible en: http://www.ncbi.nlm.nih.gov/pubmed/23642499

  15. Pérez Pérez De Prado N. La antropometría materna del segundo trimestre de la gestación y su relación con el peso del recién nacido [tesis]. Santa Clara: Universidad de Ciencias Médicas de Villa Clara; 2014.

  16. Núñez Martínez I, María Morán J, Peña FJ. Two-step cluster procedure after principal component analysis identifies sperm subpopulations in canine ejaculates and its relation to cryoresistance. J Androl [internet]. 2013 [citado 25 Enero 2015]; 27(14):[aprox.3 p]: Disponible en: http://onlinelibrary.wiley.com/doi/10.2164/jandrol.05153/full

  17. Triana de la Paz I, López Pérez R, García Gutiérrez MB, Santana Machado A, Fleites Vizcaíno MC, Sánchez Freire P. Caracterización morfométrica de espermatozoides en pacientes con espermograma normal (resultados preliminares). Medicent Electrón [internet]. 2015 sep.-dic. [citado 4 jul. 2016];19(4):[aprox. 8 p.]. Disponible en: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1029-30432015000400003&lng=es

  18. Aulesa C, Cabrera M, Alonso R, Benítez M, Martínez M. Evaluación del sistema automatizado sperm class analyzer (SCA) para análisis del semen. Rev Lab Clín [internet]. 2009 ene.-mar. [citado 13 abr. 2014]; 2(1):[aprox. 2 p.]. Disponible en: http://www.elsevier.es/sites/default/files/elsevier/pdf/282/282v02n01a13132692pdf001.pdf

  19. Ros Santaella J. Morfometría espermática en ciervo ibérico (cervus elaphus hispanicus): implicaciones biológicas y crio-biológicas [tesis]. Albacete, España: Universidad de Castilla-La Mancha; 2012.

  20. Álvarez Lleó C. Análisis integrado de morfología y movilidad espermática humana con el uso del sperm class analyzer [tesis]. Valencia, España: Universidad de Valencia; 2003 [citado 22 ene. 2015]. Disponible en: http://www.tdx.cat/bitstream/handle/10803/9486/TESI_DOC.PDF?sequence=1




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