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ISSN 1025-0255 (Electronic)

2016, Number 5

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AMC 2016; 20 (5)

Gram and urease for the diagnosis of Helicobacter pylori in gastroduodenal samples

López RM, Aties LL, Sánchez OET, Mariño CMC, Duret GY

Language: Spanish
References: 15
Page: 488-495
PDF size: 607.32 Kb.


ABSTRACT

Background: gastroduodenal affection and gastric cancer are diseases which are more frequent every day. Helicobacter pylori is the responsible microorganism for the largest number of cases.
Objective: to determine the presence of helicobacter pylori in grastroduodenal biopsy samples from the application of microbiological techniques: urease test and Gram staining.
Methods: in 2012 a cross-sectional descriptive study was conducted to determine the presence of helicobacter pylori in gastroduodenal biopsy samples through microbiological techniques in the microbiology laboratory of “Saturnino Lora” hospital in Santiago de Cuba.
Results: from the w hole group of patients studied a predominance of 98 women that account for 66.6 % was found. From the bad habits assessed the one with the highest percentage was coffee consumption, with 132 cases which represent 89,8 %. The most notable symptom found was epigastralgia. After undergoing the referred tests of the study (urease test and Gram staining), 66,7 % of positive samples with urease test and 79,6 % with Gram staining were obtained.
Conclusions: morbidity prevailed in female sex in the 31 to 45 age group. Gram staining is more sensitive than urease test. Patients with chronic gastritis showed gram-positive results to helicobacter pylori.


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