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Órgano Oficial de la Asociación Dental Mexicana

2017, Number 1

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Rev ADM 2017; 74 (1)

Bacterial identification on acrylic resin surfaces

Coronado MLP, Tinoco CVC, Méndez MR, Cornejo PMA, Escalante BSA

Language: Spanish
References: 26
Page: 40-45
PDF size: 215.30 Kb.


ABSTRACT

Introduction: The material most commonly used to make full or partial prosthetic dental appliances is polymethyl methacrylate, which provides a solid surface that is in close contact with the patient’s oral mucosa. During the production of the dental prosthetic, defects such as holes, cracks, and other irregularities can appear on this surface (depending on the method used), which act as reservoirs that stimulate the proliferation of microorganisms and make it easier for these to adhere to the surface. The most frequently isolated microorganism in denture wearers is Candida albicans. Objective: To identify the bacteria present on the acrylic resin surface of dentures bases (ProBase Hot®, Ivoclar, Vivadent). Material and methods: 10 subjects of both sexes aged between 25 and 30 years were selected from among patients attending a prosthodontics clinic. Alginate impressions of the patients were used to create plaster molds, which were then used to construct heat-cured acrylic resin palatal plates that the patients wore for a period of 24 hours. A sample of the acrylic was subsequently taken for bacteriological identification purposes. Statistical analysis was performed based on a descriptive analysis of frequency distribution and percentages, and crossover and multiple response tables created (IBM SPSS STATISTICS 21.0 software). Results: The most frequently identified bacterium in this study was Klebsiella pneumoniae, while the least frequently isolated were Escherichia coli and Enterobacter cloacae (once each), Pseudomonas aeruginosa (in three cases), and Enterococcus faecalis, Streptococcus alpha hemolytic and Streptococcus hyicus (two each). Conclusions: The acrylic resin used in this study tested positive for various bacterial species, the most frequently isolated of these belonging to the family Enterobacteriaceae.


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