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Órgano Oficial de la Asociación Dental Mexicana

2017, Number 2

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Rev ADM 2017; 74 (2)

Descriptive histological study of the colonization of bacteria in the dentinal tubules of extracted teeth with pulp necrosis

Ávila VS, Rosas GG, Pujana GSJA, Rosas BN, Llamosas HE

Language: Spanish
References: 15
Page: 69-73
PDF size: 1184.61 Kb.


ABSTRACT

Background: One of the main goals of endodontic treatment is to achieve the elimination of resident microorganisms in the root canal. However, the microorganisms involved in the pulp necrosis adapt to the conditions of necrotic canals, penetrating the dentinal tubules, which complicates treatment. Objective: The purpose of this research was to histologically describe the areas of formation and distribution of biofilm in both the canals and the dentinal tubules of teeth extracted with pulp and periapical pathology. Material and methods: 34 samples of teeth with periapical lesions were studied. None of the specimens had prior canal treatment, endoperiodontal injury, fracture nor longitudinal root fracture. Teeth were decalcified with 5% formic acid and buffered with formalin for 7 weeks. Histological routine procedure for including samples in paraffin was conducted. Longitudinal serial sections were obtained of the pulp canal space for submission to staining with hematoxylin and eosin, peryodic acid Schiff, methenamine silver, and Gram & Taylor Brown-Brenn, to identify dentinal tubules and the presence of fungi and bacteria. Results: Of the 544 histological sections under study 75% (405) showed microbial colonization. No evidence of fungi was found. 194 histological sections (35.6%) had microorganisms penetrating the dentinal tubules to a depth of 150 microns, and 211 histological sections (38.7%) had microorganisms penetrating the dentinal tubules for more than 500 µm. Conclusions: There are a large number of bacteria that manage to penetrate the dentinal tubules in the root canals of necrotic teeth. This should be taken into account when root canal treatment procedures are performed.


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