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ISSN 0864-0289 (Print)

2016, Number 4

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Rev Cubana Hematol Inmunol Hemoter 2016; 32 (4)

Correlation between morphology and flow cytometry in Acute Lymphoblastic Leukemia (ALL)

Marsán SV, del Valle PLO, Díaz DG, Macías AC, Machín GS, Lam DRM, Cháguez LO, Valdés SC, García CMB, Plá TMJ

Language: Spanish
References: 27
Page: 483-493
PDF size: 168.92 Kb.


ABSTRACT

Introduction: Acute lymphoblastic leukemia (ALL) is 75 to 80 % of all acute leukemias in childhood.
Objective: To stablish correspondance between morphologic and immnunologic, by flow cytometry (FCM), criteria in a group of children with acute leukemia (AL) and severe pancytopenia.
Methods: Between January 2008 and May 2015 a study with children with AL, from different Oncology and Hematological services of the country was carried out. The cell bone marrow immunophenotyping was performed FCM.
Results: O f these, 150 were ALL for FCM. B cell -ALL was diagnosed in 111 (74 %) patients, and T- ALL in 39 (26 %) with phenotype T -ALL. In 122 patients (81.3 %) there was correspondence between the morphology of lymphoid blasts and diagnosis of ALL, by FCM. In 28 patients no correlation between morphological and immune diagnosis was found. In 26 of these children, morphology did not identify the leukemic cell line and the diagnosis of ALL was performed exclusively by FCM. In a patient where the morphology of blasts showed features of myeloid precursors, the FCM showed expression of immature antigens of lymphoid precursors and concluded as a pro-B ALL. In a patient with severe pancytopenia, pre-T ALL was diagnosed by FCM.
Conclusions: These results demonstrate that CFM is a highly sensitive and specific technique, not only to define the leukemic cell line but also to identify those subtypes of difficult morphological diagnosis, allowing the introduction of specific anti-leukemic treatment.


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