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2017, Number 1

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Rev Ciencias Médicas 2017; 21 (1)

Comparative test between Ag inactivated macro-agglutination and in vivo Ags agglutination in the diagnosis of leptospirosis

Cabezas AH, Fernández BY, Cabezas ML

Language: Spanish
References: 15
Page: 54-61
PDF size: 277.39 Kb.


ABSTRACT

Introduction: there are several methods for the serological diagnosis of leptospirosis but more research is needed to achieve an efficient, rapid and economical way that satisfies the diagnosis.
Objective: to evaluate polyvalent Ag for macroscopic diagnosis of leptospirosis versus micro-agglutination.
Method: a polyvalent Leptospira Ag and the dilution for sera were prepared at Leptospirosis Center of Pinar del Rio in the University of Medical Sciences, and tested with sera from patients arriving at the Provincial Laboratory of Microbiology; 430 sera, 260 of suspect and leptospirosis positive patients, 120 out of 10 family practices, 20 positive for brucellosis, 15 positive for syphilis and 15 hyper-immune sera were compared between macro and micro-agglutination sera. The sera were diluted 1:80, for macro-agglutination with their dilution and for 1: 100 micro-agglutination with saline solution.
Results: the results were processed by comparison of proportions with 95% of confidence interval, estimating sensitivity and specificity. Of the suspected sera, they were positive to micro-agglutination (2.63% and 3.21% to macro-agglutination), there was no non-significant difference. Positive to Brucellaand syphilis were negative to Leptospira and the positive hyper-immune, in general specificity and sensitivity of polyvalent Leptospira Ag was 99.73 and 99.95% with respect to 100% of micro-agglutination.
Conclusions: there was no significant difference between micro-agglutination and macro-agglutination (P ≥ 0.05). Both the specificity and sensitivity of AgLP were excellent. The trials must be extended to different population groups. The method is very valuable.


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