Relova D, Pérez LJ, Coronado L, Hinojosa Y, Ríos L, Acevedo AM, Frías MT, Perera CL

Language: English
References: 0
Page: 1301-1305
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ABSTRACT

There is now widespread use of molecular tools for diagnosis and study of viral diseases. For this, it is essential to preserve the quality of genetic material while it is stored. Most laboratories store nucleic acid at ultra-low temperatures. However, there is a growing tendency on the search for new alternatives that allow storage of nucleic acid at room temperature. In this work the thermal stability of viral RNA retained as a dry pellet inside a sealed polyethylene tube and stored at 4, 20 and 37 ºC for a period of two months was studied. The study was conducted through periodic quantification of the concentration of total RNA of classical swine fever virus (CSFV) and avian influenza virus (AIV) using the rRT-qPCR. The results showed the efficacy of preservation method used in this experiment. In each time evaluated it was possible to quantify the amplification of the 5’UTR and NS5B regions of CSFV and M and HA genes of avian influenza virus, regardless of the temperature at which the RNA samples were stored. It showed that viral RNA storage as dry pellet retained within a hermetically sealed polyethylene tube is an effective way to preserve the stability of the viral RNA for long periods at room temperature.