García-Arroyo A, Rosas-Dossetti M, Santoyo-Sánchez A, Salcedo-Roldán M, Ramos-Peñafiel CO

Language: Spanish
References: 9
Page: 563-571
PDF size: 504.22 Kb.

ABSTRACT

Background: The 2009 influenza pandemic renewed interest in timely identification of suspected influenza cases through routine laboratory studies, the most studied is lactic dehydrogenase (DHL).
Objetive: To determine if patients with influenza A (H1N1) pneumonia present particular alterations inside routine laboratory studies, particularly in DHL levels and analyze the prognostic implication.
Material and Method: A case-control study of patients with confirmed diagnosis of influenza A (H1N1) pneumonia (case), and patients with bacterial pneumonia (control) treated from December 2013 to July 2014.
Results: Thirty-one cases were analyzed, 45% (n = 14) had a diagnosis of influenza A (H1N1) pneumonia, the remaining 55% (n = 17) was considered of bacterial etiology. The mean age was 38 (16-62) years old. The DHL level at diagnosis time was on average 578.77 IU/L (191-1096), higher in the group with influenza A (H1N1) pneumonia (573 IU/L vs 624.7 IU/L, p = 0.366). In the overall analysis, the levels of DHL › 350 IU/L at diagnosis time and at the end of treatment had a negative impact on mortality (OR: 84.0, 95%CI: 4.4754-1576.6044, and OR: 154.0, 8.6261-2749.3255). Overall survival was 18 days, lower in the A (H1N1) group (4 vs 25 days, p = 0.016).
Conclusions: DHL › 350 IU/L can be considered a severity biomarker, also has a negative impact on the survival of patients with pneumonia without being able to discriminate the possible etiological agent.

REFERENCES

1. Rani R, Kumar V. Recent Update on human lactate dehydrogenase enzyme 5 (hLDH5) inhibitors: A promising approach for cancer chemotherapy. J Med Chem 2015;59(2):487-96.

2. Lu R, Jiang M, Chen Z, Xu X, et al. Lactate dehydrogenase 5 expression in non-Hodgkin lymphoma is associated with the induced hypoxia regulated protein and poor prognosis. PLoS One 2013;8(9):e74853.

3. Emery AE. The determination of lactate dehydrogenase isoenzymes in normal human muscle and other tissues. Biochem J 1967;105(2):599-604.

4. Fountain JA, Parks ME, Dickey A, McKee RW. Lactate dehydrogenase isoenzymes in tissues of normal and Ehrlich-Lettré ascites tumor-bearing Swiss mice. Cancer Res 1970;30(4):998-1002.

5. Vanderlinde RE. Measurement of total lactate dehydrogenase activity. Ann Clin Lab Sci 1985;15(1):13-31.

6. Buonocore R, Avanzini P, Aloe R, Lippi G. Analytical imprecision of lactate dehydrogenase in primary serum tubes. Ann Clin Biochem 2015;53(Pt 3):405-8.

7. Kelly R, Greiff D. The level of lactic dehydrogenase activity as an indicator of the grwoth of influenza virus in the embryonate egg. J Exp Med 1961;113:125-9.

8. Prahoveanu E, Petrescu A, Moisa I. The effect of ATP on lactic dehydrogenase (LDH) activity and influenza B virus multiplication. Virologie 1975;26(1):51-5.

9. Mori S, Watanabe W, Shigeta S. A colorimetric LDH assay for the titration of infectivity and the evaluation of anti-viral