2006, Number 2
Development of antisera to identify M-1 and M-3 Streptococcus pyogenes strain serotypes associated to severe invasive infections
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ABSTRACTIntroduction: Development of antisera to identify M-1 and M-3 S. pyogenes strain serotypes is important to identify those isolated from patients with severe invasive infections in Mexico. Methods: New Zealand rabbits were immunized with M-1 and M-3 S. pyogenes strains. The immune sera was adsorbed with heterologous serotypes and challenged against homologous and heterologous strains (B3264, M-non-typeable, M-4, M-6 and M-12) using capillary precipitation test, double diffusion Ouchterlony test or indirect bactericidal test to evaluate serotype specificity and bactericidal activity. Results: The micro precipitin capillary test showed high level titers of serotype specific antibodies. The bactericidal activity of antisera against homologous strains was elevated, but absent with heterologous strains. The double diffusion Outcherlony test first showed a double band of precipitation with the homologous antiserum, which was then reduced to a single band when antisera was adsorbed against a heterologous serotype. There was no cross-reactive activity of antisera against heterologous strains and the potency was high. Conclusions: The animal model was successful for obtaining serotype specific antisera against M-1 and M-3 serotypes, without cross-reactions against other serotypes, and was also highly potent. It will be useful for identifying S. pyogenes serotypes associated with severe infections in our country.
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