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Ginecología y Obstetricia de México

Federación Mexicana de Ginecología y Obstetricia, A.C.
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2019, Number 07

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Ginecol Obstet Mex 2019; 87 (07)

Identification of vaginal microorganisms of patients in preterm labor

Gómez-Garzón M, Hernández-Yesid G, Cruz-Hernández CL, Pérez-Lozada JT, Rodríguez-Preval N, Torres-Tobar L
Full text How to cite this article

Language: Spanish
References: 43
Page: 436-446
PDF size: 455.71 Kb.


Key words:

Preterm labor, Microorganisms, Staphylococcus aureus, Ureaplasma urealyticum, Mycoplasma hominis, Clindamycin, Erythromycin.

ABSTRACT

Objective: Determine the frequency of microorganisms present in the vagina of women in preterm labor.
Materials and Methods: Descriptive, prospective, cross-sectional study of a series of cases of 46 patients treated at the Fundación Hospital Infantil Universitario de San José de Bogotá for preterm labor, who were sampled from the vaginal introitus and planted on the A.F. Genital System-Liofilchem ® panel. Genital System by Liofilchem®, according to the manufacturer's instructions. The statistical package Stata version 13 (StataCorp®) was used. The statistical analysis was descriptive, the nonparametric Wilcoxon test was run.
Results: The most isolated microorganism was Staphylococcus aureus with a frequency of 89.13%. Ureaplasma urealyticum was detected in 43.48% and Mycoplasma hominis in 19.57 Of the positive samples for genital Mycoplasmas, 52.2% showed a concentration ›105 CFU/mL. Ureaplasma urealyticum isolates showed 100% resistance to clindamycin and 100% Mycoplasma hominis for erythromycin.
Conclusions: Microorganisms that have been identified as risk factors for preterm delivery were identified in 93.5% of the vaginal discharge samples. For Ureaplasma urealyticum and Mycoplasma hominis, 100% resistance for clindamycin and erythromycin is identified.


REFERENCES

  1. OMS. Nacimientos prematuros. Datos y Cifras de 2018. https://www.who.int/es/news-room/fact-sheets/detail/ preterm-birth.

  2. Liu L, et al. Global, regional, and national causes of under-5 mortality in 2000-15: an updated systematic analysis with implications for the Sustainable Development Goals. Lancet 2016;388(10063):3027-3035. https://doi.org/10.1016/ S0140-6736(16)31593-8

  3. Blencowe H, et al. Born too soon: the global epidemiology of 15 million preterm births. Reprod Health. 2013;10(Suppl 1):S2-S2. https://doi.org/10.1186/1742-4755-10-S1-S2

  4. DANE, Información para todos. Cuadro 8. Nacimientos por tiempo de gestación según departamento, municipio y área de residencia de la madre. 2017. Dirección URL: .

  5. Romero R, et al. The vaginal microbiota of pregnant women who subsequently have spontaneous preterm labor and delivery and those with a normal delivery at term. Microbiome 2014;2:18. https://doi.org/10.1186/2049-2618-2-18

  6. Stout MJ, et al. Early pregnancy vaginal microbiome trends and preterm birth. Am J Obstet Gynecol 2017;217(3):356. e351-356.e318. https://doi.org/10.1186/2049-2618-2-18

  7. Hyman RW, et al. Diversity of the vaginal microbiome correlates with preterm birth. Reprod Sci 2014;21(1):32-40. https://doi.org/10.1177/1933719113488838

  8. Cunningham F, et al. Williams. Obstetricia: McGraw-Hill Interamericana; 2015.

  9. Acosta-Terriquez J, et al. Prevalencia de infección de vías urinarias en pacientes hospitalizadas con amenaza de parto pretérmino. Ginecol Obstet Mex 2014;82(11):737- 743. https://ginecologiayobstetricia.org.mx/secciones/ articulos-originales-numero83/prevalencia-de-infeccionde- vias-urinarias-en-pacientes-hospitalizadas-con-amenaza- de-parto-pretermino/

  10. Ugalde-Valencia D, et al. Infecciones del tracto genital y urinario como factores de riesgo para parto pretérmino en adolescentes. Rev Chil Obstet Ginecol 2012;77:338-341. http://dx.doi.org/10.4067/S0717-75262012000500003

  11. Kramer MS, et al. Stress pathways to spontaneous preterm birth: the role of stressors, psychological distress, and stress hormones. Am J Epidemiol. 2009;169(11):1319-1326. https://doi.org/10.1093/aje/kwp061

  12. Kramer MS, et al. Maternal stress/distress, hormonal pathways and spontaneous preterm birth. Paediatr Perinat Epidemiol 2013;27(3):237-246. https://doi.org/10.1111/ ppe.12042

  13. Kemp MW. Preterm birth, intrauterine infection, and fetal inflammation. Front Immunol 2014;5(574). https://doi. org/10.3389/fimmu.2014.00574

  14. Romero R, et al. A fetal systemic inflammatory response is followed by the spontaneous onset of preterm parturition. Am J Obstet Gynecol 1998;179(1):186-193.

  15. Goldenberg RL, et al. Intrauterine infection and preterm delivery. T N Engl J Med 2000;342(20):1500-1507. https:// doi.org/10.1056/NEJM200005183422007

  16. Romero R, et al. Infection and prematurity and the role of preventive strategies. Semin Neonatol 2002;7(4):259-274.

  17. Vadillo-Ortega F, Beltrán Montoya J, Zaga Clavellina V. Infección intrauterina y nacimiento pretérmino. Rev Invest Clín 2004;56:93-102. http://www.scielo.org.mx/scielo. php?script=sci_arttext&pid=S0034-83762004000100013

  18. Góngora-Rodríguez A. Estudio retrospectivo en el diagnóstico de Mycoplasma y Ureaplasma en muestra seminal de 89 pacientes en la Ciudad de México. Rev Fac Med (Méx.) 2015;58:5-12. http://www.scielo.org.mx/scielo. php?script=sci_arttext&pid=S0026-17422015000100005

  19. Oleiwi H. Rapid detection of microbial profile among women with vaginitis in Hilla City, Iraq. JAPS 2017;7(2):228- 232. https://doi.org/10.7324/JAPS.2017.70232

  20. Liofilchem AF Genital System. Sistema de búsqueda, cálculo y antibiograma gérmenes patógenos urogenitales2011. http://www.liofilchem.net/login/pd/pi/79156_PI.pdf

  21. Abdel Rahman SM, et al. Antimicrobial susceptibility pattern of genital Mycoplasmas among a group of pregnant women. Alexandria J Med 2016;52(4):353-358. https:// doi.org/10.1016/j.ajme.2015.12.005

  22. Villanueva-Egan LA, et al. Perfil epidemiológico del parto prematuro. Ginecol Obstet Mex 2008;76(9):542- 548. https://ginecologiayobstetricia.org.mx/secciones/ articulos-de-revision/perfil-epidemiologico-del-partoprematuro/

  23. Nadeau HC, et al. Infection and preterm birth. Semin Fetal Neonatal Med. 2016;21(2):100-105. https://doi. org/10.1016/j.siny.2015.12.008

  24. Laibl VR, et al. Clinical presentation of community-acquired methicillin-resistant Staphylococcus aureus in pregnancy. Obstet Gynecol 2005;106(3):461-465. https://doi. org/10.1097/01.AOG.0000175142.79347.12

  25. Washam M, et al. Risk factors for methicillin-resistant Staphylococcus aureus colonization in the neonatal intensive care unit: A systematic review and meta-analysis. Am J Infect Control 2017;45(12):1388-1393. https://doi. org/10.1016/j.ajic.2017.06.021

  26. Top KA, et al. Predictors of Staphylococcus aureus rectovaginal colonization in pregnant women and risk for maternal and neonatal infections. J Pediatr Infect Dis Soc 2012;1(1):7-15. https://doi.org/10.1093/jpids/pis001

  27. Nadisauskiene R, et al. Endocervical pathogens in women with preterm and term labour. Gynecol Obstet Invest 1995;40(3):179-182. https://doi.org/10.1159/000292331

  28. Kempley S, et al. Antibiotic treatment duration and prevention of complications in neonatal Staphylococcus aureus bacteraemia. The Journal of hospital infection. 2015;91(2):129-135. https://doi.org/10.1016/j. jhin.2015.07.002.

  29. Sorano S, et al. Chorioamnionitis caused by Staphylococcus aureus with intact membranes in a term pregnancy: A case of maternal and fetal septic shock. J Infect Chemother 2016;22(4):261-264. https://doi.org/10.1016/j. jiac.2015.10.012

  30. Doster RS, et al. Staphylococcus aureus infection of human gestational membranes induces bacterial biofilm formation and host production of cytokines. J Infect Dis 2017;215(4):653-657. https://doi.org/10.1093/infdis/ jiw300

  31. Mendoza ROE, et al. Infección del recién nacido. Camino desde la corioamnionitis hasta la sepsis neonatal. 1ª ed. Colombia: Fundación Universitaria de Ciencias de la Salud; 2012.

  32. Carroll SG, et al. Lower genital tract swabs in the prediction of intrauterine infection in preterm prelabour rupture of the membranes. Br J Obstet Gynaecol 1996;103(1):54-59.

  33. Azizmohammadi S. Antimicrobial susceptibility patterns of ureaplasma urealyticum and Mycoplasma hominis isolated from pregnant women. Iranian Red Crescent Med J 2015;17(12):e17211. https://doi.org/10.5812/ircmj.17211

  34. Kwak DW, et al. Co-infection with vaginal Ureaplasma urealyticum and Mycoplasma hominis increases adverse pregnancy outcomes in patients with preterm labor or preterm premature rupture of membranes. J Matern Fetal Neonatal Med 2014;27(4):333-337. https://doi.org/10.31 09/14767058.2013.818124.

  35. Cortese F, et al. Early and late infections in newborns: Where do we stand? A review. Pediatr Neonatol 2016;57(4):265- 273. https://doi.org/10.1016/j.pedneo.2015.09.007

  36. Seyyed EZ, et al. Group B Streptococci investigation in preterm labors. Med Arch (Sarajevo, Bosnia and Herzegovina) 2013;67(2):124-125.

  37. Ministerio de Salud y Protección Social de Colombia. Guías de Práctica Clínica para la prevención, detección temprana y tratamiento de las complicaciones del embarazo, parto o puerperio; 2013:623. https://www.minsalud.gov.co/sites/ rid/Lists/BibliotecaDigital/RIDE/INEC/IETS/Gu%C3%ADa. completa.Embarazo.Parto.2013.pdf

  38. Krohn MA, et al. Vaginal colonization by Escherichia coli as a risk factor for very low birth weight delivery and other perinatal complications. J Infect Dis 1997;175(3):606-610.

  39. Gravett MG, et al. Preterm labor associated with subclinical amniotic fluid infection and with bacterial vaginosis. Obstet Gynecol 1986;67(2):229-237.

  40. Ali GY, et al. Prevalence of Candida colonization in preterm newborns and VLBW in neonatal intensive care unit: role of maternal colonization as a risk factor in transmission of disease. J Matern Fetal Neonatal Med 2012;25(6):789-795. https://doi.org/10.3109/14767058.2011.622005

  41. Bustos BRO, et al. Infecciones por Candida en neonatología. Rev Chil Pediatr 2006;77:254-258. http://dx.doi. org/10.4067/S0370-41062006000300004

  42. Kacerovský PM, Tosner J. Preterm premature rupture of the membranes and genital mycoplasmas. Acta Medica 2009;52(3):117-120.

  43. Redelinghuys MJ, et al. Antimicrobial susceptibility patterns of Ureaplasma species and Mycoplasma hominis in pregnant women. BMC Infect Dis 2014;14:171-171. https://doi. org/10.1186/1471-2334-14-171




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Ginecol Obstet Mex. 2019;87