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Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Autónoma de Yucatán

2019, Number 3

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Rev Biomed 2019; 30 (3)

Evaluación de la citotoxicidad de nanogeles de polivinilpirrolidona (PVP) en fibroblastos murinos

García-Rodríguez LM, Díaz-Jiménez D, Perez-Guevara OL, Ges-Naranjo A, Rapado-Paneque M, Ojalvo-Garcia A, Verhe-Tamayo M, Martínez-Plous Y, Garcia-Hernandez L, Rivera-Tapia JA

Language: Spanish
References: 18
Page: 131-136
PDF size: 594.62 Kb.


ABSTRACT

Introduction.Nanogels are extensively studied for diverse biomedical applications. One of the relevant is the use as drugs nano-carriers for therapeutic purposes increasing the bioactivity and transport of active components to specific sites or cells.
Objective.The aim of this study was to evaluate the cytotoxicity of 30 nm and 90 nm PVP nanogels in murine fibroblast cells.
Methods. Cytotoxicity was evaluated through lysosomal integrity by neutral red uptake assay in murine fibroblast cell line L-929.
Results. Cytotoxicity results by neutral red uptake assay showed that there are no cytotoxic effects in cells treated with the 30 nm nanogels, at the concentrations of 50, 100, 200 and 300 µg / mL for 24 h and 48 h. However, 90 nm nanogels at 48 h presented certain cytotoxicity when the percent survival was less than 70; this effect was not observed at 24 h.
Conclusion. PVP nanogels were obtained by gamma radiation with an average size of 30 nm and 90 nm approximately. The absence of cytotoxicity of 30 nm PVP nanogels on the L-929 cell line makes them potential candidates for biomedical applications.


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