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2019, Number 1

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AbanicoVet 2019; 9 (1)

Effect of egg yolk on sperm cryopreservation of drone (Apis mellifera)

Loeza-Concha H, Domínguez-Rebolledo Á, Copas-Medina K, Vivas-Rodríguez J, Escalera-Valente F, Ramón-Ugalde J

Language: Spanish
References: 37
Page: 1-11
PDF size: 487.51 Kb.


ABSTRACT

The aim of this study was to evaluate the effect of whole yolk (WEY) and centrifuged yolk (CEY) and its interaction with dimethylsulfoxide (DMSO) and glycerol, on the cryopreservation of drone semen. Six aliquots of semen were obtained, which were divided into four treatments: T1 (CEY+ glycerol), T2 (CEY + DMSO), T3 (WEY + glycerol) and T4 (WEY + DMSO). The diluted samples were evaluated after 10 minutes of incubation at 37 °C and after thawing. Motility was evaluated subjectively (scale 4 to 0), membrane integrity (Host test) and viability (IP/ SYBR-14). The results were analyzed with ANOVA. At the time of dilution, T1 was the highest percentage of motility, viability and integrity of the membrane presented (grade 4, 95.75% ± 1.20, 89.75% ± 0.42, respectively), after thawing the T2 was the highest percentage presented (grade 2, 59.10% ± 1.44, 84.35% ± 0.72, respectively). The results showed that T1 was the one that obtained the best sperm parameters at the time of dilution, while, after thawing, T2 was the diluent that best cryopreserved sperm samples from drone.


REFERENCES

  1. ABOAGLA EME, Terada T. 2004. Effects of egg yolk during the freezing step of cryopreservation on the viability of goat spermatozoa. Theriogenology. 62 (6), 1160- 1172.DOI: 10.1016/j.theriogenology.2004.01.013

  2. BURLEY LM, Fel, RD, Saacke, RG. 2008. Supervivencia de espermatozoides de miel de abeja (Hymenoptera: Apidae) incubados a temperatura ambiente de drones expuestos a Miticidas. Diario de Entomología Económica, 101 (4), 1081-1087. DOI: 10.1093 / jee / 101.4.1081

  3. BRIAND-AMIRAT L, Tainturier D, Anton M. 2007. Use of egg compounds for cryoprotection of spermatozoa. In Bioactive egg compounds. Springer, Berlin, Heidelberg. 259-264. ISBN: 13:978-3-540-37883. Disponible en: https://link.springer.com/chapter/10.1007%2F978-3-540-37885-3_30

  4. CABRERA F, Gonzalez F, Batista M, Calero P, Medrano A, Gracia A. 2005. The effect of removal of seminal plasma, egg yolk level and season on sperm freezability of canary buck (Capra hircus). Reproduction in domestic animals. 40(3):191-195. DOI: 10.1111/j.1439-0531.2005.00544.x

  5. COBEY S, Sheppard WS, Tarpy DR. 2012. Status of breeding practices and genetic diversity in domestic US honey bees. Honey bee colony health: challenges and sustainable solutions. CRC Press, Boca Raton. Pp. 25-36. ISBN-13: 978-1439879405 Disponible En: https://www.researchgate.net/publication/303873161_Status_of_breeding_practices_an d_genetic_diversity_in_domestic_US_honey_bees

  6. COLLINS AM. 2004. Functional longevity of honey bee, Apis mellifera, queens inseminated with low viability semen. Journal of Apicultural Research. 43: 167-171. DOI: 10.1080 / 00218839.2004.11101131

  7. COLLINS AM, Pettis JS. 2001. Effect of Varroa infestation on semen quality. American Bee Journal. 141 (8), 590. Disponible en: https://www.ars.usda.gov/research/publications/publication/?seqNo115=118617

  8. COLLINS AM, Donoghue AM. 1999. Viability assessment of honey bee, Apis mellifera, sperm using dual fluorescent staining. Theriogenology, 51(8):1513-1523. DOI: 10.1016/S0093-691X(99)00094-1

  9. COLLINS AM. 2000. Relationship between semen quality and performance of instrumentally inseminated honey bee queens. Apidologie. 31(3): 421-429. DOI: 10.1051/apido:2000132

  10. DADKHAH F, Nehzati-Paghaleh G, Zhandi M, Emamverdi M, Hopkins BK. 2016. Preservation of honey bee spermatozoa using egg yolk and soybean lecithin-based semen extenders and a modified cryopreservation protocol. Journal of Apicultural Researc. 55(4):279-283. DOI: 10.1080/00218839.2016.1243292

  11. FERNÁNDEZ-SANTOS MR, Esteso MC. Montoro V, Soler AJ, Garde JJ. 2006. Cryopreservation of Iberian red deer (Cervus elaphus hispanicus) epididymal spermatozoa: effects of egg yolk, glycerol and cooling rate. Theriogenology. 66(8):1931- 1942. DOI: 10.1016/j.theriogenology.2006.05.012

  12. GUZMÁN-NOVOA E, Eccles L, Calvete Y, Mcgowan J, Kelly PG, Correa-Benítez A. 2010. Varroa destructor is the main culprit for the death and reduced populations of overwintered honey bee (Apis mellifera) colonies in Ontario, Canada. Apidologie. 41:443-450. DOI: 10.1051/apido/2009076

  13. HARBO JR. 1983. Survival of honey bee (Hymenoptera: Apidae) spermatozoa after two years in liquid nitrogen (–196 C). Annals of the Entomological Society of America. 76(5): 890-891.DOI: 10.1093/aesa/76.5.890

  14. HOPKINS BK, Herr C. 2010. Factors affecting the successful cryopreservation of honey bee (Apis mellifera) spermatozoa. Apidologie. 41:548-556. DOI: 10.1051/apido/20010006

  15. HOPKINS BK, Baker PJ, Sheppard, WS, Herr C. 2011. The importance of egg-yolk in the cryopreservation of honey bee (Apis mellifera) semen. Cryobiology. 3(63):333. DOI: 10.1016/j.cryobiol.2011.09.102

  16. HOPKINS BK, Herr C, Sheppard WS. 2012. Sequential generations of honey bee (Apis mellifera) queens produced using cryopreserved semen. Reproduction, Fertility and Development. 24(8):1079-1083.DOI: 10.1071/RD11088

  17. HU J, Li Q, Zan L, Jiang Z, An J, Wang L, Jia Y. 2010. The cryoprotective effect of lowdensity lipoproteins in extenders on bull spermatozoa following freezing–thawing. Anim Reprod Sci. 117:11-17. DOI: 10.1016/j.anireprosci.2009.04.001

  18. JIAN-HONG H, Zhong-Liang J, Rui-Kai L, Qing-Wang L, Shu-Shan Z, Lin-Sen Z, Yao Kun L, Xin L. 2011. The advantages of low-density lipoproteins in the cryopreservation of bull semen. Cryobiology. 62: 83-87. DOI: 10.1016/j.cryobiol.2010.12.007

  19. KARGER S, Geiser B, Grau M, Burfeind O, Heuwieser W, Arlt SP. 2016. Prognostic value of a pre-freeze hypo-osmotic swelling test on the post-thaw quality of dog semen. Animal reproduction science. 166:141-147. DOI: 10.1016/j.anireprosci.2016.01.015

  20. KULAKSIZ R, Çebi Ç, Akçay E, Daşkın A. 2010. The protective effect of egg yolk from different avian species during the cryopreservation of Karayaka ram semen. Small ruminant research. 88(1):12-15. DOI: doi.org/10.1016/j.smallrumres.2009.11.014

  21. LAIDLAW H, Lorenzen C. 1977. Laidlaw instrumental insemination instrument [Queen honey bees]. American Bee Journal (USA). Disponible en: http://agris.fao.org/agrissearch/ search.do?recordID=US7728540

  22. LOCKE SJ, Peng YS. 1993. The effects of drone age, semen storage and contamination on semen quality in the honey bee (Apis mellifera). Physiological entomology. 18:144- 148. DOI: 10.1111/j.1365-3032.1993.tb00461.x

  23. LENSKY Y, Schindler H. 1967. Motility and reversible inactivation of honeybee spermatozoa in vivo and in vitro. Les Annales de l'Abeille, 10 (1), 5-16. DOI: 10.1051/apido:19670101

  24. MEDEIROS A, Gomes G, Carmo M, Papa FO, Alvarenga MA. 2002. Cryopreservation of stallion sperm using different amides. Theriogenology. 58 (2): 273-276. DOI: 10.1016/S0093-691X(02)00898-1

  25. MOUSSA M, Martinet V, Trimeche A, Tainturier D, Anton M. 2002. Low density lipoproteins extracted from hen egg yolk by an easy method: cryoprotective effect on frozen-thawed bull semen. Theriogenology. 57:1695-1706. DOI: 10.1016/S0093- 691X(02)00682-9

  26. NEUMANN P, Carreck NL. 2015. Honey bee colony losses. Journal of Apicultural Research, 49:1-6. DOI: 10.3896/IBRA.1.49.1.01

  27. NOURI H, Towhidi A, Zhandi M, Sadeghi R. 2013. The effects of centrifuged egg yolk used with INRA plus soybean lecithin extender on semen quality to freeze miniature Caspian horse semen. Journal of equine veterinary science. 33:1050-1053. DOI: 10.1016/j.jevs.2013.03.184

  28. NUR Z, Seven-Cakmak S, Ustuner B, Cakmak I, Erturk M, Abramson CI, Sağirkaya H, Soylu MK. 2012. The use of the hypo-osmotic swelling test, water test, and supravital staining in the evaluation of drone sperm. Apidologie. 43:31-38. DOI: 10.1007/s13592- 011-0073-1

  29. PILLET E, Duchamp G, Batellier F, Beaumal V, Anton M, Desherces S, Schmitt E, Magistrini M. 2011. Egg yolk plasma can replace egg yolk in stallion freezing extenders. Theriogenology. 75:105-114. DOI: 10.1016 / j.theriogenology.2010.07.015 SPSS, IIBM (2011). Estadísticas de IBM SPSS para Windows, versión 20.0. Nueva York: IBM Corp,440

  30. STORNELLI MC, Tittarelli CM, Savignone CA, Stornelli MA. 2005. Efecto de los procesos de criopreservación sobre la fertilidad seminal. Analecta veterinaria. 25. Disponible en: http://sedici.unlp.edu.ar/bitstream/handle/10915/11180/Documento_completo.pdf?seque nce=1&isAllowed=y

  31. TAYLOR MA, Guzman-Novoa E, Morfin N, Buhr MM. 2009. Improving viability of cryopreserved honey bee (Apis mellifera L.) sperm with selected diluents, cryoprotectants, and semen dilution ratios. Theriogenology. 72:149-59. DOI: 10.1016/j.theriogenology.2009.02.012

  32. TOFILSKI A. 2014. A scientific note on amoeboid movement of honey bee semen. Apidologie. 45(5) 637-640. DOI: 10.1007 / s13592-014-0269-2

  33. TENTCHEVA D, Gauthier L, Zappu lla N, Dainat B, Cousserans F, Colin ME, Bergoin M. 2004. Prevalence and seasonal variations of six bee viruses in Apis mellifera L. and Varroa destructor mite populations in France. Applied and environmental microbiology, 70:7185-7191. DOI: 10.1128 / AEM.70.12.7185-7191.2004

  34. VANENGELSDORP D, Hayes Jr J, Underwood RM, Pettis JS. 2010. A survey of honey bee colony losses in the United States, fall 2008 to spring 2009. Journal of apicultural research. 49:7-14. DOI: 10.3896/IBRA.1.49.1.03

  35. WEGENER J, Bienefeld K. 2012. Toxicity of cryoprotectants to honey bee semen and queens. Theriogenology. 77:600–607. DOI: 10.1016/j.theriogenology.2011.08.036

  36. WATSON PF, Martin ICA. 1975. The influence of some fractions of egg yolk on the survival of ram spermatozoa at 5oC. Australian journal of biological sciences. 28(2):145- 152. DOI: 10.1071/BI9750145

  37. WATSON, PF. 2000. Las causas de la reducción de la fertilidad con semen crioconservado. Ciencia de la reproducción animal, 60, 481-492. DOI: 10.1016/S0378- 4320(00)00099-3




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