medigraphic.com
ISSN 1561-3070 (Electronic)

2019, Number 4

<< Back Next >>

Rev Cub Oftal 2019; 32 (4)

Characterization of basal membrane, nervous plexus and corneal thickness by confocal microscopy in diabetic patients

Gutiérrez CM, Castillo PAC, Ramos LM, Pérez PZ, Ramos PY, Barroso LR

Language: Spanish
References: 11
Page: 1-11
PDF size: 395.39 Kb.


ABSTRACT

Objective: To identify the morphological and morphometric characteristics of the corneal layers by confocal microscopy in diabetic patients.
Methods: A descriptive and comparative study was carried out in 90 eyes, 60 of which belonged to diabetic patients (30 type 1 and 30 type 2) and 30 to supposedly healthy patients. The study was conducted at Ramón Pando Ferrer Cuban Institute of Ophthalmology, between January 2012 and January 2017.
Results: Epithelial thickness, stroma and corneal endothelium was greater in the eyes of type 1 diabetics with a mean of 54.6, 506.7 and 26.7 microns, respectively. The epithelial basement membrane was observed in 20% of the eyes of patients with type 1 diabetes mellitus, and in 10% of type 2 diabetics.
The group of type 1 diabetics showed one and two nerve plexuses per confocal microscopy field for 33.3% each. In type 2 diabetics, one nerve plexus (40%) predominated, while in healthy patients, four nerve plexuses (66.7%) predominated. Both groups of diabetic patients presented tortuous vertical plexuses (40% and 53.3%, respectively) and oblique nerve plexuses in the group of supposedly healthy patients (80%).
Conclusions: The study of the cornea by confocal microscopy in diabetic patients showed greater total and layered corneal thicknesses, visible basal membrane, decrease in the sub-basal nerve plexus with vertical arrangement, and tortuous fiber.


REFERENCES

  1. Jurado I. Fase teórica: Ceguera. Documento; 2019 [acceso: 25/10/2019]. Disponible en: https://www.scribd.com/document/216409932/Fase-Teorica-ceguera-docx

  2. Dirección Nacional de Registros Médicos y Estadísticas de Salud. Anuario Estadístico de Salud. La Habana: MINSAP; 2016:4.

  3. Weston BC, Bourne WM, Polse WB, Hodge DO. Corneal hydration control in Diabetes Mellitus. Rochester, Minnesota: Mayo Clinic; 2005. p. 38-41.

  4. Caixinha M, Oliveira P, Aires ID, Ambrósio AF, Santiago AR, Santos M, Santos J. In vivo characterization of corneal changes in a type 1 diabetic animal model. Ultrasound Med Biol. 2019;45(3):823-32.

  5. Deardorff PM, McKay TB, Wang S, Ghezzi CE, Cairns DM, Abbott RD, et al. Modeling Diabetic Corneal Neuropathy in a 3D In Vitro Cornea System. 􀀶􀁆􀁌􀁈􀁑􀁗􀀃􀀵􀁈􀁓􀀑􀀃2018;8(1):17294.

  6. Sang Beom H, Hee Kyung Y, Joon Young H. Influence of diabetes mellitus on anterior segment of the eye. Clin Interv Aging. 2019;14:53-63.

  7. Deák EA, Szalai E, Tóth N, Malik RA, Berta A, Csutak A. Longitudinal Changes in Corneal Cell and Nerve Fiber Morphology in Young Patients with Type 1 Diabetes with and without Diabetic Retinopathy: A 2-Year Follow-up Study. Invest Ophthalmol Vis Sci. 2019;60:830-7.

  8. Ishibashi F, Taniguchi M, Kosaka A, Uetake H, Tavakoli M. Improvement in neuropathy outcomes with normalizing HbA(1c) in patients with type 2 diabetes. Diab Care. 2018;42:110- 8.

  9. Mocan M, Durukan I, lrkek M, Orhan M. Morphologic alterations of both the stromal and subbasal nerves in the corneas of patients with diabetes. Cornea. 2006;25(7):769-73.

  10. Liu XW, Pang GX, Wang Z. ln vivo confocal microscopy observation and evaluation of sensation in diabetic corneas. 􀀽􀁋􀁒􀁑􀁊􀁋􀁘􀁄􀀃􀀼􀁄􀁑􀀃􀀮􀁈􀀃􀀽􀁄􀀃􀀽􀁋􀁌􀀑􀀃2005;41(10):920-3.

  11. Midena E, Brugin E, Ghirlando A, Sommavilla M, Avogaro A. Corneal diabetic neuropathy: a confocal microscopy study. J Refract Surg. 2006;22 (Suppl.):47-52.




2020     |     www.medigraphic.com