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Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Autónoma de Yucatán

2021, Number 2

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Rev Biomed 2021; 32 (2)

Anti-proliferative eff ect in breast and cervical-uterine cancer cell lines, caused by multi-walled carbon nanotubes - lectin of Phaseolus lunatus var. silvester Baudet

Aguilar-Vázquez J, Pina-Canseco MS, Gochi-Ponce Y, Pérez-Santiago AD

Language: Spanish
References: 49
Page: 87-97
PDF size: 1005.46 Kb.


ABSTRACT

Introduction. Carbon nanotubes have been conjugated with a wide range of molecules for use in medicine due to the properties they have, such as size, chemical and mechanical properties, and they can be targeted at specific sites.
Objective. To evaluate the cytotoxic eff ect of the multiple-walled carbon nanotube conjugate with lectin de Phaseolus lunatus (NTCPM-lPl) in four carcinoma cell lines (MDA MB-231, MCF-7, ViBo and HeLa).
Methods. Multiple-walled carbon nanotube (NTCPM) were purified in a mixture of concentrated acids, 1: 3 [HNO3 (70%) / H2SO4 (95-98%)] and sonicated for 4 h, the pH was adjusted to 7 and incubated with lectin of Phaseolus lunatus var. silvester (lPl) for 4 h under constant stirring at 4° C. The cytotoxic activity of IPI and the NTCPM-IPI conjugate was measured by MTT and crystal violet tests at 24, 48 and 72 h, in two carcinoma cell lines.
Results. Synchronized ViBo, MDA MB-231 and MCF-7 cells showed a reduction of 51, 53 and 47% respectively with the NTCPM-lPl conjugate, while HeLa showed a 55% reduction with lPl alone.
Conclusions. Cytotoxic activity is potentiated using the NTCPM-lPl conjugate in synchronized ViBo, MDA MB-231 and MCF-7 cells; however, for HeLa cells the eff ect is produced by lPl alone, so conjugation is unnecessary.


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