>Medicina Cutánea Ibero-Latino-Americana
>Year 2003, Issue 5
Lacueva L, Hausmann G, Ferrando J
Expression of Cutaneous Lymphocyte Antigen and E-Selectin in Alopecia Areata
Med Cutan Iber Lat Am 2003; 31 (5)
PDF: 309.93 Kb.
As we recently reported, the cutaneous lymphocyte associated antigen (CLA) is highly expressed in the patches of alopecia areata (AA) of the scalp (1). The aim of our study is to determinate the CLA expression in the cellular components of the infiltrate at different levels: intradermic, intrafollicular, perifollicular and perivascular; as well as to determine the E-Selectin expression in the dermal vascular endothelium.
Material and Methods. Twelve biopsies of the scalp corresponding to 12 patients affected of AA as well as 8 biopsies of the occipital region of healthy controls were performed.
a) CLA expression: A double directe immunofluorescence technique (were performed in all samples, with the primary mononuclear antibodies: anti-CD1a, anti-CD4, anti-CD8, anti-CD68 and HECA-452, and two fluorescent secondary antibodies versus IgG and IgM, following the double staining technique with FITC and Rodamine.
b) E-Selectin expression: In all samples a Ulex staining technique followed by the E-Selectin expression with the Envision technique of Dako were performed.
Results. CLA expression were differently manifested by the cellular components. HECA-452 were positive in 58% of the cells of the AA biopsies, while healthy scalp biopsies expressed HECA-452 only in 10% of cells. In AA the highest expression of CLA were manifested by T lymphocytes, similar within CD4+ (71%) and CD8 (70%) cells. The perifollicular area manifested the highest expression of CLA (61% of cells). Concerning to the E-Selectin, 7 out of the 12 samples of AA showed within 79-97% of cellular expression of that component, while in healthy scalp samples it was manifested within 29-55%.
Conclusions. Perifollicular T lymphocytes mainly shows CLA expression and probably follicular target structures in AA are located in the most external components of the hair follicle. May be the different expression of CLA by CD4+ and CD8+ cells, in the different areas, gives the key to the specific infiltrate as well as the pathogenesis of this disorder. E-Selectin-CLA interaction may play a primordial role in the migratory process in that and others inflammatory dermatosis.
||alopecia areata, E-Selectin, cutaneous lymphocyte antigen.
Lacueva L, Hausmann G, Ferrando J. Expresión del antígeno linfocitario cutáneo (CLA) en las placas alopécicas de pacientes con alopecia areata. Med Cutan Iber Lat Am 2003; 31: 161-72.
Bos JD, de Boer OJ, Tibbosch E, Das PK, Pal ST. Skin-homing T lymphocytes: detection of cutaneous lymphocyte associated antigen (CLA) by HECA-452 in normal skin. Arch Dermatol Res 1993; 285: 179-83.
Picker LJ, Mitchie SA, Rott LS, Butcher EC. A unique phenotype of skin-associated lymphocytes in humans. Preferential expression of the HECA-452 epitope by benigne and malignnant T cell cutaneous sites. Am J Pathol 1990; 136: 1053-68.
Picker LJ, Martin RJ, Trumble A, Newman LS, Collins PA, Bergtresser PR et al. Differential expression of lymphocyte homing receptors by human memory/effector T cell in pulmonary versus cutaneous immune effector site. Eur J Immunol 1994; 24: 1269-77.
Rossiter H, van Reijsen F, Mudde GC, Kalthoff F, Bruijnzeel KC, Picker LJ, Kupper TS. Skin disease-related T cells bind to endothelial selectins. Expression of cutaneous lymphocyte antigen (CLA) predits E-Selectin but non P-Selectin binding. Eur J Immunol 1994; 24: 205-10.
Picker LJ, Treer JR, Ferguson DB, Collins PA, Bergstresser PR, Terstappen LW. Control of lymphocyte recirculation in man. II. Differential regulation of the cutaneous lymphocyte-associated antigen, a tissue-selective homing receptor for skin-homing T cell. J Immunol 1993; 150: 1122-36.
Koszik F, Strunk D, Simonitsch I, Picker LJ, Stingl G, Payer E, Expression of monoclonal antibody HECA-452 defined E-Selectin ligands on Langerhans cells in normal and disease skin. J Invest Dermatol 1994; 102: 773-80.
Unger RE, Yawalkar N, Braathen LR y Brand CU. The HECA-452 epitope is highly expressed on lymph cells derived from human skin. Br J Dermatol 1999; 141: 565-9.
Nakamura K, Yasaka N, Asahina A, Kato M, Miyazono K, Furne M, Tamaki K. Increased numbers of CD68 antigen positive dendritic epidermal cells and upregulation of CLA expression on these cells in various skin diseases. J Dermatol Sci 1998; 18: 170-80.
Kieffer JD, Fuhlbrigge RC, Armerding D, Robert C, Ferenczi K, Camphausen RT, Kupper TS. Neutrophils, monocytes and dendritic cells express the same specialized form of PSGL-1 as do skin-homing memory T cells: CLA. Biochem Biophys Res Common 2001; 285: 577-87.
Yoshino T, Okano M, Chen HL, Tsuchiyama J, Kondo E, Nishiuchi R, Teramoto N, Nishizaki K, Akagi T. Cutaneous lymphocyte antigen is expressed on memory/effector B cells in the peripheral blood and monocytoid B cells in the limphoid tissues. Cell Immunol 1999; 197: 39-45.
Berg EL, Yoshino T, Rott LS, Robinson MK, Warnoock RA, Kishimoto TK et al. The cutaneous lymphocyte antigen is a skin lymphocyte homing receptor for the vascular lectin endothelial cell-leukocyte adhesion molecule 1. J Exp Med 1991; 174: 1461-6.
Berg EL, Robinson MK, Mansson O, Butcher EC, Magnani JL. A carbohydrate domain common to both sialyl Le-a and sialyl Le-x is recognized by the endothelial cell leukocyte adhesion molecule, ELAM-1. J Biol Chem 1991; 266: 14869.
Springer T, Allison JP, Murphy KM, Griffiths G, Paulnock DM. Inmnidad mediada por células T (8): 263-365. En: Janeway C A Jr. Inmunobiología. Ed. Msson. Barcelona 2000 4ª edición.
Ghersetich I, Campanile G, Lotti T. Alopecia areata: Immunohistochemistry and ultrastructure of infiltrate and identification of adhesion molecule receptors. Int J Dermatol 1996; 35: 28-33.
>Medicina Cutánea Ibero-Latino-Americana
>Year 2003, Issue 5