Muñoz-Guzmán MA, Alba-Hurtado F

Idioma: Español/Inglés
Referencias bibliográficas: 24
Paginas: 59-64
Archivo PDF: 138.86 Kb.

RESUMEN

El propósito del presente trabajo fue determinar los antígenos de secreción-excreción de larvas de Toxocara canis (Ag-SETc) que son más frecuentemente reconocidos por cachorros infectados naturalmente del área metropolitana de la Ciudad de México. A la necropsia, 20 cachorros presentaron fases adultas de T. canis en el intestino (64.6%) y 11 no presentaron (35.4%). El suero de todos los animales con T. canis en el intestino y de siete animales, sin gusanos a la necropsia, fueron positivos a ELISA (X = 0.683 ± 0.184 DO) y reconocieron, por Western blot (WB), varios Ag-SETc. El suero de cuatro cachorros sin T. canis en el intestino no reconoció ningún antígeno por WB y fueron negativos a ELISA-IgG (X = 0.078 ± 0.045 DO). Los sueros de los cachorros reconocieron tres grupos de antígenos. El primero incluyó siete de bajo peso molecular (16, 20, 23, 24, 28, 32 y 38 kDa); el segundo, tres de alto peso molecular (400, 200 y 120 kDa) y el tercero antígenos de peso molecular intermedio (86, 74, 66 y 47 kDa). Los Ag-SETc más frecuentemente reconocidos fueron de 32, 38, 66, 120 y 200 kDa, por lo que éstos son candidatos para pruebas de inmunodiagnóstico en perros.

REFERENCIAS (EN ESTE ARTÍCULO)

1. ABO-SHEHADA MN, AL-ZUBAIDY BA, HERBERT IV. The migration of larval Toxocara canis in mice. I. Migration through the intestine in primary infections.Vet Parasitol 1984;17:65-73.

2. ALBA-HURTADO F, TORTORA PJ, TSUTSUMI V, ORTEGA-PIERRES M.G. Histopathological investigation of experimental ocular toxocariasis in gerbils. Int J Parasitol 2000;30:143-147

3. FINSTERER J, AUER H. Neurotoxocarosis. Rev Inst Med Trop Sao Paulo 2007; 49:279-287.

4. MAGNAVAL JF, GLICKMAN LT, DORCHIES P, MORASSIN B. Highlights of human toxocariasis. Kor J Parasitol 2001;39:1-11.

5. MAIZELS RM, DE SAVIGNY D, OGILVIE BM. Characterization of surface and excretory-secretory antigens of Toxocara canis infective larvae. Parasite Immunol 1984;6 :23-37.

6. BADLEY JE, GRIEVE RB, BOWMAN DD, GLICKMAN LT, ROCKEY JH. Analysis of Toxocara canis larval excretory-secretory antigens: physicochemical characterization and antibody recognition. J Parasitol 1987;73:593-600.

7. MEGHJI M, MAIZELS RM. Biochemical properties of larval excretory-secretory glycoproteins of the parasitic nematode Toxocara canis. Mol Biochem Parasitol 1986;18:155-170.

8. SARIMEHMETOGLU HO, BURGU A, AYCICEK H, GÖNENÇ B, TANYUKSEL M, KARA M. Application of western blotting procedure for the immunodiagnosis of visceral larva migrans in mice by using excretory/secretory antigens. Dtsch Tierarztl Wochenschr 2001;108:390-392.

9. SOMMERFELT IE, SANTILLAN G, LOPEZ C, RIBICICH M, FRANCO AJ. Immunological and hematological response in experimental Toxocara canis-infected pigs. Vet Parasitol 2001;96:127-134.

10. MORALES OL, LOPEZ MC, NICHOLLS RS, AGUDELOC. Identification of Toxocara canis antigens by Western blot in experimentally infected rabbits. Rev Inst Med Trop Sao Paulo 2002;44:213-216.

11. ALBA-HURTADO F, MUÑOZ-GUZMAN MA, VALDIVIA-ANDA G, TORTORA JL, ORTEGA-PIERRES M.G. Toxocara canis: larval migration dynamics, detection of antibody reactivity to larval excretory-secretory antigens and clinical findings during experimental infection of gerbils (Meriones unguiculatus). Exp Parasitol 2009 IN PRESS.

12. SAVIGNY DH. In vitro maintenance of Toxocara canis larvae and a simple method for the production of Toxocara ES antigen for use in serodiagnostic tests for visceral larva migrans. J Parasitol 1975;61:781-782.

13. BOWMAN DD, MIKA-GRIEVE M, GRIEVE RB. Circulating excretory-secretory antigen levels and specific antibody responses in mice infected with Toxocara canis. Am J Trop Med Hyg 1987;36:75-82.

14. JOHANSSON KE. Separation of antigens by analytical gel electrophoresis. In: BJERRUM OJ, HEEGAARD NH, editors. Handbook of immunoblotting of proteins. Vol. I Florida:CRC-Press, 1988:31-50.

15. BRADFORD MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 1976;72:248-254.

16. COLIGAN JE, KRUISBEEK AM, MARGULIES DH, SHEVACH EM, STROBER W. editors. Immunoblotting and immunodetection, Current protocols in immunology Vol 2. New York:Wiley & Sons, 1994.

17. PARK HY, LEE SU, HUH S, KONG Y, MAGNAVAL JF. A seroepidemiological survey for toxocariasis in apparently healthy residents in Gangwon-do, Korea. Kor J Parasitol 2002;40:113-117.

18. EGUIA-AGUILAR P, CRUZ-REYES A, MARTINEZ-MAYA JJ. Ecological analysis and description of the intestinal helminths present in dogs in Mexico City. Vet Parasitol 2005;127:139-146.

19. HABLUETZEL A, TRALDI G, RUGGIERI S, ATTILI AR, SCUPPA P, MARCHETTI R et al. An estimation of Toxocara canis prevalence in dogs, environmental egg contamination and risk of human infection in the Marche region of Italy. Vet Parasitol 2003;113:243-252.

20. MARTINEZ-MORENO FJ, HERNANDEZ S, LOPEZCOBOS E, BECERRA C, ACOSTA I, MARTINEZMORENO A. Estimation of canine intestinal parasites in Cordoba (Spain) and their risk to public health. Vet Parasitol 2007;143:7-13.

21. FAHRION AS, STAEBLER S, DEPLAZES P. Patent Toxocara canis infections in previously exposed and in helminth-free dogs after infection with low numbers of embryonated eggs. Vet Parasitol 2008;152:108-115.

22. ISHIDA MM, RUBINSKY-ELEFANT G, FERREIRA AW, HOSHINO-SHIMIZU S, VAZ AJ. Helminth antigens (Taenia solium, Taenia crassiceps, Toxocara canis, Schistosoma mansoni and Echinococcus granulosus) and cross-reactivities in human infections and immunized animals. Acta Trop 2003;89:73-84.

23. PERTEGUER MJ, CUELLAR C, GUILLÉN JL, AGUILA C, FENOY S, CHIVATO T et al. Cross-reactivity between Anisakis simplex sensitization and visceral larva migrans by Toxocara canis. Acta Trop 2003;89:85-89. 24. MAGNAVAL JF, FABRE R, MAURIERES P, CHARLET JP, DE LARRARD B. Application of the western blotting procedure for the immunodiagnosis of human toxocariasis. Parasitol Res 1991 ;77 :697-702.

24. MAIZELS RM, PAGE AP. Surface associated glycoproteins from Toxocara canis larval parasites. Acta Trop 1990 ;47 :355-364