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Instituto Nacional de Salud Pública

2021, Número 1

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salud publica mex 2021; 63 (1)


Patotipos diarreicos de Escherichia coli aislados de una granja porcina en una región del estado de Morelos, México

Tamayo-Legorreta E, García-Radilla A, Moreno-Vázquez E, Téllez-Figueroa F, Alpuche-Aranda CM

Idioma: Ingles.
Referencias bibliográficas: 34
Paginas: 34-41
Archivo PDF: 415.17 Kb.


RESUMEN

Objetivo. Determinar la frecuencia de patotipos diarreicos de Escherichia coli que colonizan cerdos. Material y métodos. Se analizaron cepas de E. coli aisladas de muestras fecales de 280 cerdos, producidas para consumo local, en una granja del estado de Morelos (centro de México) para identificar los patotipos diarreicos de E. coli por PCR multiplex. Se determinó la electroforesis en gel de campo pulsado (PFGE) para el estudio de la diversidad genética entre cepas patógenas de E. coli. Resultados. De los 521 aislados de E. coli con patotipos diarreicos examinados, 50 (9.6%) fueron positivos para al menos un gen de virulencia en 42 animales diferentes. Así, 15% (42/280 porcinos) fueron colonizados con E. coli patógeno. Entre los aislados de E. coli de porcinos, el patotipo EPEC (6.5%) fue el más frecuente, seguido por EHEC (2.3%), ETEC y EIEC (0.4%). El estudio de la diversidad genética, realizado por PFGE de 40 cepas representativas, reveló 25 perfiles de restricción distintos agrupados en 21 grupos (A-U). Conclusiones. En este estudio, se encontraron cuatro diferentes patotipos de E. coli entre los cerdos colonizados por E. coli. Estos cerdos son reservorios de estas bacterias virulentas y existe un riesgo potencial de causar diarrea en los cerdos y en la población que consume la carne.


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