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2024, Number 78

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Oral 2024; 25 (78)

Antimicrobial action of pediatric mouthwashes on Streptococcus mutans strains in comparison with chlorhexidine, in vitro study

Mayorga-Villalón DI, González-Guerrero CE, de la Garza-Ramos M, Reyes-Sepúlveda JE
Full text How to cite this article

Language: Spanish
References: 12
Page: 2310-2315
PDF size: 332.78 Kb.


Key words:

dental caries, mouthwashes, chlorhexidine.

ABSTRACT

The purpose of this study was to determine the antimicrobial action of Colgate ® Plax Kids and Equate ® Kids mouthwashes, compared to chlorhexidine. The present study was experimental in vitro, using two methods to verify the effectiveness of the mouthwashes. In the first method, paper discs impregnated with the rinse solutions were placed in Petri dishes where the strains of Streptococcus mutans (S. mutans) were planted in a trypticase soy agar medium, incubated by 24 h at 37 oC, obtaining as a result halos and percentage of bacterial inhibition of the Colgate ® Plax Kids 1 mm, Equate ® Kids 2 mm and chlorhexidine 0.5 mm mouthwash samples. An ANOVA statistical analysis was performed, obtaining a value of p = 0.490, which indicates that there is no statistically significant difference. The second experiment was carried out in a 96-wells microplate where the strain of S. mutans and the different types of mouthwashes mixed with brain-heart infusion medium and artificial salive were placed. The quantification of bacteria was compared in the different treatment groups where the Colgate ® Plax Kids mouthwash showed the greatest reduction. Additionally, the Dunnett test was performed, which showed a statistically greater difference in the mouthwashes studied (p ‹ 0.05) compared to the chlorhexidine. It is concluded that S. mutans is sensitive to mouthwashes based on cetylpyridinium and chlorhexidine, being even more sensitive to mouthwashes that do not contain chlorhexidine.


REFERENCES

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Oral. 2024;25