2024, Number 78
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Oral 2024; 25 (78)
Antimicrobial action of pediatric mouthwashes on Streptococcus mutans strains in comparison with chlorhexidine, in vitro study
Mayorga-Villalón DI, González-Guerrero CE, de la Garza-Ramos M, Reyes-Sepúlveda JE
Language: Spanish
References: 12
Page: 2310-2315
PDF size: 332.78 Kb.
ABSTRACT
The purpose of this study was to determine the antimicrobial action of Colgate
® Plax Kids and Equate
® Kids mouthwashes, compared
to chlorhexidine. The present study was experimental in vitro, using two methods to verify the effectiveness of the mouthwashes. In the
first method, paper discs impregnated with the rinse solutions were placed in Petri dishes where the strains of Streptococcus mutans (S.
mutans) were planted in a trypticase soy agar medium, incubated by 24 h at 37
oC, obtaining as a result halos and percentage of bacterial
inhibition of the Colgate
® Plax Kids 1 mm, Equate
® Kids 2 mm and chlorhexidine 0.5 mm mouthwash samples. An ANOVA statistical analysis
was performed, obtaining a value of p = 0.490, which indicates that there is no statistically significant difference. The second experiment
was carried out in a 96-wells microplate where the strain of S. mutans and the different types of mouthwashes mixed with brain-heart
infusion medium and artificial salive were placed. The quantification of bacteria was compared in the different treatment groups where the
Colgate
® Plax Kids mouthwash showed the greatest reduction. Additionally, the Dunnett test was performed, which showed a statistically
greater difference in the mouthwashes studied (p ‹ 0.05) compared to the chlorhexidine. It is concluded that S. mutans is sensitive to
mouthwashes based on cetylpyridinium and chlorhexidine, being even more sensitive to mouthwashes that do not contain chlorhexidine.
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