medigraphic.com
SPANISH

Gaceta Médica de México

ISSN 0016-3813 (Print)
  • Contents
  • View Archive
  • Information
    • General Information        
    • Directory
  • Publish
    • Instructions for authors        
  • medigraphic.com
    • Home
    • Journals index            
    • Register / Login
  • Mi perfil

2008, Number 2

<< Back Next >>

Gac Med Mex 2008; 144 (2)

VI. La biología molecular en el diagnóstico micológico en México

Hernández-Hernández F
Full text How to cite this article

Language: Spanish
References: 8
Page: 134-136
PDF size: 35.72 Kb.


Key words:

Opportunistic mycosis, molecular biology.

ABSTRACT

In the last 20 years the molecular biology has reached all the medicine areas. The medical mycology has not been the exception and one of its major objectives is the early identification of the etiological agents of fungal infections. The polymerase chain reaction (PCR) has been the molecular technique most widely used. However in Mexico the development of these procedures is yet limited mainly because it requires highly specialized staff and the high costs. Therefore, the laboratories which have succeeded in the standarization of this technique and offer it as a part of the hospital services are very few. Infections which get more attention are systemic mycosis caused by low virulence fungi such as Candida, Aspergillus, Cryptococcus, Pneumocystis and some mucorales species, due to the high mortality in patients with severe risk factors. Some of these species are difficult to identify by non molecular procedures such as culture and histopathology which are time consuming, so the establishment of these methods in the mycological diagnosis laboratories is necessary.


REFERENCES

  1. Bodey G, Bueltmann B, Duguid W, Gibas D, Hanak H, Hotchi M, et al. Fungal infections in cancer patients: an international autopsy survey. Eur J Clin Microbiol Infect Dis 1992;11:99-109.

  2. Méndez-Tovar LJ, López-Martínez R, Macotela-Ruiz E, Manzano-Gayosso P, Serrano-Jaen L, Carmona-Castañón A, et al. Variación en la frecuencia de micosis en México. Rev Argent Microbiol 1999;31:107-103.

  3. Karthaus M, Cornely OA. Recent developments in the management of invasive fungal infections in patients with hematological malignancies. Ann Hematol 2005;84:207-216.

  4. García-Ruiz JC, Amutio E, Pontón J. Infección fúngica invasora en pacientes inmunodeficientes. Rev Iberoam Micol 2004;21:55-62.

  5. Einsele H, Hebart H, Roller G, Löffler J, Rothenhofer I, Müller CA, et al. Detection and identification of fungal pathogens in blood by using molecular probes. J Clin Microbiol 1997;35:1353-1360.

  6. Pinto PM, Resende MA, Koga-Ito CY, Ferreira JA, Tendler M. rDNA-RFLP identification of Candida species in immunocompromised and seriously diseased patients. Can J Microbiol 2004;50:514-520.

  7. Ramírez-Gaona AY. Identificación fenotípica y genotípica de aislados de Sporothrix schenckii obtenidos de la naturaleza en el estado de Puebla. Tesis de Maestría, Centro de Investigaciones en Ciencias Microbiológicas, Benemérita Universidad Autónoma de Puebla, México, 2005.

  8. Baires-Varguez L, Cruz-García A, Villa-Tanaka L, Sánchez-García S, Gaitán-Cepeda LA, Sánchez-Vargas LO, et al. Comparison of a randomly amplified polymorphic DNA (RAPD) analysis and ATB ID 32C system for identification of clinical isolates of different Candida species. Rev Iberoam Micol 2007;24:148-151.




2020     |     www.medigraphic.com

Mi perfil

C?MO CITAR (Vancouver)

Gac Med Mex. 2008;144